AMB  >> Vol. 4 No. 1 (March 2015)

    不同培养基配方对美花石斛生根培养的影响
    Effect of Different Culture Medium Formulas on Dendrobium loddigesii Rolfe Root Culture

  • 全文下载: PDF(326KB) HTML   XML   PP.6-10   DOI: 10.12677/AMB.2015.41002  
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作者:  

周 路,罗国庆,扬 风,汪建华,王自布:贵州师范学院化学与生命科学学院,贵州 贵阳

关键词:
石斛组织培养生根培养美花石斛Dendrobium Stem Tissue Culture Root Growth Dendrobium loddigesii Rolfe

摘要:

目的:为美花石斛种苗的规模化生产提供理论依据。方法:以将继代培养的美花石斛长至0.2~0.6 cm左右的丛生芽分割外植体,分别转入不同的培养基中。研究MS培养基浓度,激素(NAA)浓度对美花石斛组培苗生根的影响。结果:MS培养基的浓度对美花石斛组培苗生根影响较大,组培苗在1/2 MS培养基中有明显的促进作用。在培养基中添加激素(NAA)对美花石斛组培苗生根有很大的促进作用,随着激素(NAA)浓度的增加,组培苗生根率越高,当NAA增加到0.7 mg/L时达到86%,再增高会出现生长缓慢,叶变枯黄。结论:当培养基配方为1/2MS + 0.7 mg/L时,美花石斛组培苗生根及生长效果最佳。

Objective: The effect of different culture medium formulas on Dendrobium loddigesii root culture was studied to provide theoretical basis for the standardization production. Method: We investi-gated the effect of media components on the induction of shoot regeneration for different types of explants to research the effect on taking root for Dendrobium loddigesii. Results: The media com-ponents have a great influence on taking root. And 1/2 MS medium can obviously promote rooting. And with the increasing of NAA, the rooting rate rises. But when the NAA increased to 0.7 mg/L, and the rooting rate reached 86% and then increased again, there will be a slow growth with the leaves turning yellow. Conclusion: The best culture medium formula is 1/2 ms + 0.7 mg/L NAA for Dendrobium loddigesii to take root and grow.

文章引用:
周路, 罗国庆, 扬风, 汪建华, 王自布. 不同培养基配方对美花石斛生根培养的影响[J]. 微生物前沿, 2015, 4(1): 6-10. http://dx.doi.org/10.12677/AMB.2015.41002

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