AMB  >> Vol. 2 No. 1 (March 2013)

    内生细菌YBS106对棉花的诱导抗性研究
    Disease-Resistance Induction in Cotton by Inoculation with Endophytic Bacteria Strains YBS106

  • 全文下载: PDF(268KB) HTML   XML   PP.16-21   DOI: 10.12677/AMB.2013.21004  
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作者:  

管芩澜,林天兴:乐山师范学院生命科学学院,乐山;
龚明福:乐山师范学院生命科学学院、峨眉山特色生物资源重点实验室,乐山

关键词:
内生细菌棉花棉花枯萎病菌棉花黄萎病菌诱导抗性Endophytic Bacteria; Cotton; Fusarium oxysporum; Verticillium dahliae; Induced Resistanc

摘要:

本实验以从一把伞南星(Arisaema erubescens (Wall.) Schott)组织分离到的内生细菌YBS106对三叶一心棉苗进行灌根诱导同时挑战接种棉花枯萎病菌或棉花黄萎病菌测定YBS106诱导对棉叶中抗性相关酶PODPPO活性及与抗性相关的MDAVC含量的影响同时测定YBS106对棉花枯、黄萎病的相对防效。结果表明YBS106诱导对PODPPO活性及MDAVc含量均有显著影响。YBS106能有效诱棉叶中PODPPO等防御酶活及Vc含量增加和MDA含量的减少MDAVc含量在诱导后第5天达到最高PODPPO活性在诱导后第10天达到最高。病原菌挑战接种100YBS106对棉花枯萎病菌和黄萎病菌的相对防效分别为64.24%71.37%,这与棉花系统抗性相关的防御酶活及相关物质(MDAVc)含量的变化趋势吻合。该结果表明YBS106诱导棉花产生了对棉花枯萎病菌和棉花黄萎病菌的系统抗性。
The induced resistance of Serratia marcescens strain YBS106 isolated from Arisaema erubescens (Wall.) Schott tissue to cotton Fusarium and Verticillium wilt was studied by measuring the change of the activities of defense enzymes (POD, PPO) and the contents of pathogenesis-related biochemicals (MDA, Vc) in cotton leaf inoculation with YBS106, Fusarium oxysporum, Verticillium dahliae and the control effects of strain YBS106 against to F. oxysporum and V. dahliae in pot tests. The results indicated that YBS106 effectively increased POD and PPO activities, also promoted concentration of MDA and Vc. When the F. oxysporum or V. dahliae used with YBS106, respectively, the induced activities of POD, PPO and concentration of Vc were higher than that of only used F. oxysporum or V. dahliae, but concentration of MDA were lower. The concentration of MDA and Vc peaked 5 days after the treatment while the activities of POD and PPO reached their maximum 10 days after the treatment. The relative anti-bacterial effects of strain YBS106 against to F. oxysporum and V. dahliae in pot tests when 100 day inoculated pathogen were 64.24% and 71.37%, which is related to changes consistent trend of the defense enzymes activity and related substances (MDA and Vc) content. Systemic resistance in cotton against to Fusarium oxysporum and Verticillium dahliae could be induced by the antagonistic bacteria Serratia marcescens strain YBS106.

文章引用:
管芩澜, 林天兴, 龚明福. 内生细菌YBS106对棉花的诱导抗性研究[J]. 微生物前沿, 2013, 2(1): 16-21. http://dx.doi.org/10.12677/AMB.2013.21004

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