粒毛盘菌胞内黑色素的提取工艺、红外光谱及抗氧化活性

Extraction Technology, Infrared Spectra and Antioxidant Activity of Intracellular Melanin from Lachnum

摘要: 采用碱溶酸沉法、超声波辅助提取法以及酶解法提取粒毛盘菌YM404胞内黑色素(LIM404)，并在单因素实验的基础上，运用响应面法优化获得超声波辅助法的最佳提取条件为：NaOH浓度1.5mol·L^-1、超声功率82 W、提取温度75℃、提取时间33 min，在此条件下提取的黑色素(LIM404-b)得率最高，为12.50%，较碱溶酸沉法获得的黑色素(LIM404-a)、酶解法获得的黑色素(LIM404-c)得率分别提高了5.04%、23.76%。红外光谱显示，LIM404-b的结构特征与LIM404-a、LIM404-c的基本相似。LIM404-b浓度为500mg·L^-1时，与1.4251mmol·mL^-1 α-生育酚的总抗氧化能力相当，对·OH、DPPH·、·的清除率分别为64.43%、76.05%、68.43%，表明LIM404-b具有较强的抗氧化活性。 Intracellular melanins from Lachnum YM404 (LIM404) were obtained by the methods of alkali solution and acid isolation precipitation, ultrasonic-assisted extraction, enzyme hydrolysis, respectively. Based on the single factor experiments, Box-Behnken experiment was designed to obtain the optimum ultrasonic-assisted extraction conditions of LIM404: NaOH 1.5 mol·L^-1, ultrasonic power 82 W, ultrasonic temperature 75˚C and ultrasonic time 33 min. Under these conditions, the yield of melanin (LIM404-b) was 12.5%, and increased 5.04%, 12.15% than that by alkali solution and acid precipitation, enzyme hydrolysis, respectively. FT-IR results showed that the structural characteristics of LIM404-b were similar to that of LIM404-a, LIM404-c. When the LIM404-b concentration was 500 mg·L^-1, its total antioxidant capacity was equivalent to α-tocopherol of 1.4251 mmol·mL^-1, and the ·OH, DPPH·and · scavenging rates were 64.43%, 76.05%, 68.43%, respectively, suggesting that the antioxidant activity of LIM404-b was strong.