细菌生物膜的形成及耐药机制研究进展
Progress on Bacterial Biofilm Formation and Antibiotics Resistance Mechanisms
DOI: 10.12677/AMB.2013.24018, PDF, HTML,  被引量    科研立项经费支持
作者: 周 敏, 尤理想, 赵 青, 李嫄渊, 吴淑燕, 黄 瑞：苏州大学基础医学与生物科学学院病原生物学系，苏州
关键词: 细菌生物膜；耐药性；耐药基因；质粒；Bacterial Biofilm; Antibiotic Resistance; Drug Resistance Genes; Plasmid
摘要: 细菌对抗生素的耐药性已成为全球关注的医学及社会问题，细菌生物被膜(Bacterial biofilm, BBF)是细菌耐药性产生的一个重要方面。BBF是由细菌及其产生的胞外大分子多聚物形成的一种特殊细菌群落。这种细菌群落具有特殊的空间结构，能产生很强的屏障作用，抵抗抗生素的杀菌作用，并且可以加强群落内细菌的交流，传递耐药基因。在耐药基因的传递过程中，质粒作为基因的载体起到了极大的作用。同时，BBF与基因表达存在相互影响的关系。对BBF、耐药基因及质粒的研究，可以提高对BBF耐药性播散的认识，有助于防治细菌耐药性。 Bacterial resistance to antibiotics has become a global medical and social issue. Bacterial biofilm (BBF) is an important aspect of bacterial resistance. It is a special bacterial community formed by bacteria and their extracellular polymeric matrix. It has a special spatial structure that can produce a strong barrier to resist antibiotics, enhance the communication among bacteria and transfer the resistance genes. In the process of the transmission of resistance genes, plasmid plays a great role as gene vector. Meanwhile, BBF interacts closely with gene expression. The research on BBF, plasmid and drug resistance genes can facilitate understanding of the spread and prevention of BBF resistance.
文章引用：周敏, 尤理想, 赵青, 李嫄渊, 吴淑燕, 黄瑞. 细菌生物膜的形成及耐药机制研究进展[J]. 微生物前沿, 2013, 2(4): 98-101. http://dx.doi.org/10.12677/AMB.2013.24018

参考文献

[1] Qu, Y., Daley, A.J., Istivan, T.S., et al. (2010) Antibiotic suscep- tibility of coagulase-negative staphylococci isolated from very low birth weight babies: Comprehensive comparisons of bacteria at different stages of biofilm formation. Annals of Clinical Mi- crobiology and Antimicrobials, 9, 16.
[2] Mellata, M., Maddux, J.T., Nam, T., et al. (2012) New insights into the bacterial fitness-associated mechanisms revealed by the characterization of large plasmids of an avian pathogenic E. Coli. PLoS One, 7, e29481.
[3] Burmølle, M., Thomsen, T.R. and Fazli. (2010) Biofilms inchronic infections—A matter of opportuni-ty—Monospecies biofilms in multispecies infections. FEMS Immunology and Medical Micro- biology, 59, 324-336.
[4] O’Connell, H.A., Niu, C. and Gilbert, E.S. (2006) Enhanced high copy number plasmid maintenance and heterologous pro- tein production in an Escherichia coli biofilm. Biotechnology and Bioengineering, 97, 439-446.
[5] Moons, P., Michiels, C.W. and Aertsen, A. (2009) Bacterial interactions in biofilms. Critical Reviews in Microbiology, 35, 157-168.
[6] Lenz, A.P., Williamson, K.S., Pitts, B., et al. (2008) Localized gene expression in Pseudomonas aeruginosa biofilms. Applied and Environmental Microbiology, 74, 4463-4471.
[7] He, X. and Ahn, J. (2011) Differential gene expression in plank- tonic and biofilm cells of multiple antibiotic-resistant Salmo- nella typhimurium and Staphylococcus aureus. FEMS Microbi- ology Letters, 325, 180-188.
[8] 王欢, 刘新, 张佩等 (2012) 呼吸机导管内细菌生物被膜抗吞噬作用. 中国公共卫生, 28, 644-646.
[9] 白丹, 余加林, 万珍艳等 (2008) 铜绿假单胞菌生物膜悬液和藻酸盐对小鼠巨噬细胞吞噬功能的影响. 中国微生态学杂志, 20, 337-342.
[10] Yamaguchi, Y., Park, J.H., Inouye, M., et al. (2009) A crucial regulator for quorum sensing and biofimn formation, is a GCU- specific mRNAn interferase in Escherichia coli. The Journal of Biological Chemistry, 284, 28746-28753．
[11] 董洪燕, 彭大新, 焦新安等 (2011) 肠炎沙门氏菌鸡源株ompR基因缺失株的构建及生物学特性与亲本株的比较. 微生物学报, 51, 1256-1262.
[12] Ghigo, J.M. (2001) Natural conjugative plasmids induce bacte- rial biofilm development. Nature, 412, 442-445.
[13] 龚风云, 刘丽娜, 邢铭友等 (2011) 表皮葡萄球菌耐药与生物膜的相关研究. 中华医院感染学杂志, 21, 21-23.
[14] 王丽丽, 龚凤云, 谢旭等 (2011) pvdQ基因对铜绿假单胞菌生物膜形成的影响. 华中科技大学学报(医学版), 40, 147-150.
[15] Estrela, A.B., Heck, M.G. and Abraham, W.R. (2009) Novel approaches to control biofilm infections. Current Medicinal Chemistry, 16, 1512-l530.
[16] Jonas, S.M., Mette, B., Lars, H.H., et al. (2012) The interconnec- tion between biofilm formation and horizontal genetransfer. FEMS Immunology and Medical Microbiology, 65, 183-195.
[17] 黄瑞, 秦爱兰, 闻玉梅 (2000) 耐药质粒pRST98在不同种属肠道杆菌间的接合和表达. 中国微生物学和免疫学杂志, 20, 323-326.
[18] Sørensen, S.J., Bailey, M., Hansen, L.H., et al. (2005) Studying plasmid horizontal transfer in situ: Acritical review. Nature Re- views Microbiology, 3, 700-710.
[19] Whitchurch, C.B., Tolker-Nielsen, T., Ragas, P.C., et al. (2002) Extracellular DNA required for bacterial biofilm formation. Sci- ence, 295, 1487.
[20] Molin, S. and Tolker-Nielsen, T. (2003) Gene transfer occurs with enhanced efficiency inbiofilms and induces enhanced stabi- lization of the biofilms tructure. Current Opinion in Biotechnol- ogy, 14, 255-261.

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