尿素通道蛋白B的转运特性
Transport Characteristics of Urea Transporter-B
DOI: 10.12677/JPS.2014.21001, PDF, HTML, 下载: 3,291  浏览: 11,518  国家自然科学基金支持
作者: 钟丹丹, 杨宝学：天然药物及仿生药物国家重点实验室，北京大学基础医学院药理学系，北京
关键词: 尿素通道蛋白B；尿素；尿素类似物；NH3；水；UT-B; Urea; Urea Analogues; Ammonia; Water
摘要: 尿素通道蛋白B(urea transporters B, UT-B)主要表达在红细胞和一些上皮及内皮细胞膜，介导尿素的跨膜转运。近年来研究发现UT-B亦参与其他物质的转运，如水、NH3和尿素类似物等，提示UT-B还可以作为水和气体通道。本文主要阐述UT-B对这些物质的转运特性，并探究其可能的生理学意义。 Urea transporter UT-B is highly expressed in the membrane of erythrocytes and some epithelial and endothelial cells, in which UT-B facilitates urea transport. Recently, studies find that water, ammonia and urea analogues can also be transported by UT-B. This review mainly focuses on the transport characteristics of UT-B, and the possible physiological significance will be discussed as well.
文章引用：钟丹丹, 杨宝学. 尿素通道蛋白B的转运特性[J]. 生理学研究, 2014, 2(1): 1-4. http://dx.doi.org/10.12677/JPS.2014.21001

参考文献

[1] Shayakul, C., Clemencon B. and Hediger, M.A. (2013) The urea trans-porter family (SLC14): Physiological, pathological and structural as-pects. Molecular Aspects of Medicine, 34, 313-322.
[2] Bagnasco, S.M. (2003) Gene Structure of Urea Transporters. The American Jour-nal of Physiology-Renal Physiology, 284, F3F10.
[3] Fenton, R.A., et al. (1999) The murine urea transporter genes Slc14a1 and Slc14a2 Occur in Tandem on Chromosome 18. Cytogenetics and Cell Genetics, 87, 95-96.
[4] Hunter, F.R. (1967) Facilitated diffusion in the chloride shift in human erythrocytes. Biochimica et Biophysica Acta, 135, 784787.
[5] Macey, R.I. and Farmer, R.E. (1970) Inhibition of water and solute permeability in human red cells. Biochimica et Biophysica Acta-Biomembranes, 211, 104-106.
[6] Funder, J. and Wieth, J.O. (1974) Human red cell sodium and potassium in metabolic alkalosis. Scandinavian Journal of Clinical & Laboratory Investigation, 34, 49-59.
[7] You, G., et al. (1993) Cloning and characterization of the vasopressin-regulated urea transporter. Nature, 365, 844-847.
[8] Olives, B., et al. (1994) Cloning and functional expres-sion of a urea transporter from human bone marrow cells. Journal of Biological Chemistry, 269, 31649-31652.
[9] Yang, B., et al. (2002) Urea-selective concentrating defect in transgenic mice lacking urea transporter UT-B. Journal of Biological Chemistry, 277, 10633-10637.
[10] Bankir, L., Chen, K. and Yang, B. (2004) Lack of UT-B in vasa recta and red blood cells prevents urea-induced im-provement of urinary concentrating ability. The American Journal of Physiology-Renal Physiology, 286, F144-F151.
[11] Zhao, D., et al. (2007) Comparative transport efficiencies of urea analogues through urea transporter UT-B. Biochimica et Biophysica Acta, 1768, 1815-1821.
[12] Nakhoul, N.L., et al. (1998) Effect of expressing the water channel aquaporin-1 on the CO2 permeability of Xenopus oo-cytes. The American Journal of Physiology, 274, C543-548.
[13] Endeward, V., et al. (2006) Evidence that aquaporin 1 is a major pathway for CO2 transport across the human erythrocyte membrane. Faseb Journal, 20, 1974-1981.
[14] Holm, L.M., et al. (2005) NH3 and NH4+ permeability in aquaporin-expressing Xenopus oocytes. Pflugers Archiv-European Journal of Physiology, 450, 415-428.
[15] Herrera, M., Hong N.J. and Garvin, J.L. (2006) Aqua-porin-1 transports NO across cell membranes. Hypertension, 48, 157164.
[16] Herrera, M. and Garvin, J.L. (2007) Novel role of AQP-1 in NO-dependent vasorelaxation. The American Journal of Physiolo-gy-Renal Physiology, 292, F1443-F1451.
[17] Endeward, V., et al. (2008) RhAG protein of the Rhesus complex is a CO2 channel in the human red cell membrane. FASEB Journal, 22, 64-73.
[18] Marini, A.M., et al. (2000) The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. Nature Genetics, 26, 341-344.
[19] Fang, X., Yang, B.X., Matthay, M.A. and Verkman, A.S. (2002) Evidence against aquaporin-1-dependent CO2 permeability in lung and kidney. The Journal of Physiology, 542, 63-69.
[20] Yang, B., et al. (2000) Carbon dioxide permeability of aquaporin-1 measured in erythrocytes and lung of aquaporin-1 null mice and in reconstituted proteoliposomes. The American Journal of Physiology, 275, 2686-2692.
[21] Geyer, R.R., et al. (2013) Move-ment of NH3 through the human urea transporter B: a new gas channel. The American Journal of Physiology-Renal Physiology, 304, F1447-F1457.
[22] Eastridge, M.L., Bucholtz, H.F., Slater, A.L. and Hall, C.S. (1998) Nutrient requirements for dairy cattle of the National Research Council versus some commonly used ration software. Jour-nal of Dairy Science, 81, 3049-3062.
[23] Preston, G.M., Carroll, T.P., Guggino, W.B. and Agre, P. (1992) Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science, 256, 385-387.
[24] Yang, B. and Verkman, A.S. (1998) Urea transporter UT3 functions as an efficient water channel. Direct evidence for a common water/urea pathway. The American Journal of Physiology, 273, 9369-9372.
[25] Sidoux-Walter, F., et al. (1999) At physiological expression levels the Kidd blood group/urea transporter protein is not a water channel. The American Journal of Physiology, 274, 3022830235.
[26] Yang, B. and A.S. (2002) Verkman, Analysis of double knockout mice lacking aquaporin-1 and urea transporter UT-B. Evidence for UT-B-facilitated water transport in erythrocytes. The American Journal of Physiology, 277, 36782-36786.
[27] Azouzi, S., et al. (2013) Energetic and Molecular Water Permeation Mechanisms of the Human Red Blood Cell Urea Transporter B. PloS ONE, 12, Article ID: e82338.
[28] Levin, E.J., et al. (2012) Structure and per-meation mechanism of a mammalian urea transporter. Proceedings of the National Academy of Sciences of the United States of America, 109, 1119411199.

为你推荐