基于GC-MS的代谢组学方法筛选肺癌标志物的分析流程与研究进展
Recent Development of GC-MS-Based Analytical Procedures of Metabolomics in Screening of Lung Cancer Biomarkers
DOI: 10.12677/AAC.2014.42003, PDF, HTML, 下载: 4,141  浏览: 15,882  科研立项经费支持
作者: 李彩霞, 张佩佩, 王利超, 刘会君, 潘再法, 王丽丽：浙江工业大学化学工程学院，杭州
关键词: 气相色谱–质谱联用；代谢组学；肺癌标志物；Gas Chromatography-Mass Spectrometry (GC-MS)； Metabolomics； Lung Cancer Biomarkers
摘要: 完整的代谢组学分析流程包括样本的采集与前处理、代谢组数据的采集和获取、数据的处理与分析、标志物的识别与生物学阐释等步骤。本文依据代谢组学的分析流程综述了基于GC-MS的代谢组学方法筛选肺癌标志物的研究流程及进展，并对GC-MS分析技术在代谢组学研究中存在的问题进行了总结与展望。
Abstract: The complete analytical procedures of metabolomics include sample collection and pretreatment, metabolomic data collection and acquisition, data processing and analysis, as well as markers identification and biological interpretation. In this context, the review deals with the current development and further trends in the discovery of lung cancer biomarkers based on GC-MS analytical procedures of metabolomics.
文章引用：李彩霞, 张佩佩, 王利超, 刘会君, 潘再法, 王丽丽. 基于GC-MS的代谢组学方法筛选肺癌标志物的分析流程与研究进展[J]. 分析化学进展, 2014, 4(2): 11-19. http://dx.doi.org/10.12677/AAC.2014.42003

参考文献

[1] Chen, W.Q., Zheng, R.S., Zhang, S.W., et al. (2013) Report of incidence and mortality in China cancer registries, 2009. Chinese Journal of Cancer Research, 25, 10-21.
[2] 胡海洋, 林之峰, 林强 (2013) 肺癌早期诊断的研究进展. 现代生物医学进展, 14, 2768-2773.
[3] Nicholson, J.K., Connelly, J., Lindon, J.C., et al. (2002) Metabonomics: A platform for studying drug toxicity and gene function. Nature Reviews Drug Discovery, 1, 153-161.
[4] Miekisch, W., Schubert, J.K. and Noeldgeschomburg, G.F. (2004) Diagnostic potential of breath analysis-focus on volatile organic compounds. Clinica Chimica Acta, 347, 25-39.
[5] Buszewski, B., Kesy, M., Ligor, T., et al. (2007) Human exhaled air analytics: Biomarkers of diseases. Biomedical Chromatography, 21, 553-566.
[6] Silva, C.L., Passos, M. and Camara, J.S. (2011) Investigation of urinary volatile organic metabolites as potential cancer biomarkers by solid-phase microextraction in combination with gas chromatography-mass spectrometry. British Journal of Cancer, 105, 1894-1904.
[7] 李娟, 任路静, 孙冠男, 等 (2013) 气相色谱–质谱联用技术及其在代谢组学中的应用. 生物工程学报, 4, 434446.
[8] Bando, K., Kawahara, R., Kunimatsu, T., et al. (2010) Influences of biofluid sample collection and handling procedures on GC-MS based metabolomic studies. Journal of Bioscience and Bioengineering, 110, 491-499.
[9] 汪明明, 程海婷, 薛明 (2011) 基于LC-MS的代谢组学分析流程与技术方法. 国际药学研究杂志, 2, 130-136.
[10] Gordon, S.M., Szidon, J.P., Krotoszynski, B.K., et al. (1985) Volatile organic compounds in exhaled air from patients with lung cancer. Clinical Chemistry, 31, 1278-1282.
[11] O’neill, H.J., Gordon, S.M., O’neill, M.H., et al. (1988) A computerized classification technique for screening for the presence of breath biomarkers in lung cancer. Clinical Chemistry, 34, 1613-1618.
[12] Phillips, M., Altorki, N., Austin, J.H., et al. (2007) Prediction of lung cancer using volatile biomarkers in breath. Cancer Biomarkers, 3, 95-109.
[13] 俆凤娟 (2008) 肺部呼吸诊断特征气体的研究. 硕士论文, 浙江大学, 杭州, 47-66.
[14] 於锦 (2011) 呼出气体及其冷凝物中肺癌标志物及其检测方法的研究. 硕士论文, 浙江大学, 杭州, 23-44.
[15] 胡燕婕 (2010)肺癌呼气特征性VOCs的筛选及诊断价值研究. 博士论文, 浙江大学, 杭州, 1-52.
[16] Santonico, M., Lucantoni, G., Pennazza, G., et al. (2012) In Situ detection of lung cancer volatile fingerprints using bronchoscopic air-sampling. Lung Cancer, 77, 46-50.
[17] Matsumura, K., Opiekun, M., Oka, H., et al. (2010) Urinary volatile compounds as biomarkers for lung cancer: A proof of principle study using odor signatures in mouse models of lung cancer. PLoS ONE, 5, 1-11.
[18] Guadagni, R., Miraglia, N., Simonelli, A., et al. (2011) Solid-phase microextraction-gas chromatography-mass spectrometry method validation for the determination of endogenous substances: Urinary hexanal and heptanal as lung tumor biomarkers. Analytica Chimica Acta, 701, 29-36.
[19] Hanai, Y., Shimono, K., Matsumura, K., et al. (2012) Urinary volatile compound as biomarkers for lung cancer. Bioscience, Biotechnology, and Biochemistry, 76, 679-684.
[20] Deng, C.H., Zhang, X.M. and Li, N. (2004) Investigation of volatile biomarkers in lung cancer blood using solid-phase microextraction and capillary gas chromatography-mass spectrometry. Journal of Chromatography B, 808, 269-277.
[21] Deng, C.H., Li, N. and Zhang, X.M. (2004) Development of headspace solid-phase microextraction with on-fiber derivatization for de-termination of hexanal and heptanal in human blood. Journal of Chromatography B, 813, 47-52.
[22] Sanchez, M.D., Garcia, E.H., Pavon, J.L.P., et al. (2012) Fast analytical methodology based on mass spectrometry for the determination of volatile biomarkers in saliva. Analytical Chemistry, 84, 379-385.
[23] Zhou, X.M., He, C.C., Liu, Y.M., et al. (2012) Metabonomic classification and detection of small molecule biomarkers of malignant pleural effusions. Analytical and Bioanalytical Chemistry, 404, 3123-3133.
[24] Liu, H.J., Wang, H., Li, C.X., et al. (2014) Investigation of volatile organic metabolites in lung cancer pleural effusions by solid-phase microextraction and gas chromatography/mass spectrometry. Journal of Chromatography B, 945-946, 53-59.
[25] Wang, Y.S., Hu, Y.J., Wang, D., et al. (2012) The analysis of volatile organic compounds biomarkers for lung cancer in exhaled breath, tissues and cell lines. Cancer Biomarkers, 11, 129-137.
[26] Hanai, Y., Shimono, K., Oka, H., et al. (2012) Analysis of volatile organic compounds released from human lung cancer cells and from the urine of tumor-bearing mice. Cancer Cell International, 12, 7.
[27] Naz, S., Garcia, A. and Barbas, C. (2013) Multiplatform analytical methodology for metabolic fingerprinting of lung tissue. Analytical Chemistry, 85, 10941-10948.
[28] 蔡品品, 卢红梅 (2013) 气相色谱-质谱分析血清代谢组前处理中的除蛋白及衍生方法. 分析化学, 8, 1183-1187.
[29] 牛艳洁, 江银玲, 许长江, 等 (2012) 代谢组学方法分析肺癌患者血清和尿液小分子代谢产物的初步研究. 中国肺癌杂志, 4, 195-201.
[30] Ulanowska, A., Trawinska, E., Sawrycki, P., et al. (2012) Chemotherapy control by breath profile with application of SPME-GC/MS method. Journal of Separation Science, 35, 2908-2913.
[31] Rocha, S.M., Caldeira, M., Carrola, J., et al. (2012) Exploring the human urine metabolomic potentialities by comprehensive two-dimensional gas chromatography coupled to time of flight mass spectrometry. Journal of Chromatography A, 1252, 155-163.
[32] Dunn, W.B., Broadhurst, D., Begley, P., et al. (2011) Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nature Protocols, 6, 10601083.
[33] Wedge, D.C., Allwood, J.W., Dunn, W., et al. (2011) Is serum or plasma more appropriate for intersubject comparisons in metabolomic studies? An assessment in patients with small-cell. Analytical Chemistry, 83, 6689-6697.
[34] Wen, T., Gao, L., Wen, Z.M., et al. (2013) Exploratory investigation of plasma metabolomics in human lung adenocarcinoma. Molecular BioSystems, 9, 2370-2378.
[35] Borges, C.R., Rehder, D.S. and Boffetta, P. (2013) Multiplexed surrogate analysis of glycotransferase activity in whole biospecimens. Analytical Chemistry, 85, 2927-2936.
[36] Poli, D., Goldoni, M., Corradi, M., et al. (2010) Determination of aldehydes in exhaled breath of patients with lung cancer by means of on-fiber-derivatisation SPME-GC/MS. Journal of Chromatography B, 878, 2643-2651.
[37] Hecht, S.S., Hochalter, J.B., Carmella, S.G., et al. (2013) Longitudinal study of [D10] phenanthrene metabolism by the diol epoxide pathway in smokers. Biomarkers, 18, 144-150.
[38] Buszewski, B., Ligor, T., Jezierski, T., et al. (2012) Identification of volatile lung cancer markers by gas chromatographymass spectrometry: Comparison with discrimination by canines. Analytical and Bioanalytical Chemistry, 404, 141-146.
[39] Kischkel, S., Miekisch, W., Fuchs, P., et al. (2012) Breath analysis during one-lung ventilation in cancer patients. European Respiratory Journal, 40, 706-713.
[40] 许国旺, 等 (2008) 代谢组学—方法与应用. 科学出版社, 北京, 38-40.
[41] Kleckner, I.R. and Foster, M.P. (2012) GUARDD: User-friendly MATLAB software for rigorous analysis of CPMG RD NMR data. Journal of Biomolecular NMR, 52, 11-22.
[42] 黄欣, 龚益飞, 虞科, 等 (2007) 基于气相气谱-质谱的代谢组学方法研究四氯化碳致小鼠急性肝损伤. 分析化学, 12, 1736-1740.
[43] 赵丽丽 (2009) 代谢组学在肺癌中的临床应用研究. 硕士论文, 大连医科大学, 大连, 15-30.
[44] Tautenhahn, R., Patti, G.J., Rinehart, D., et al. (2012) XCMS online: A web-based platform to process untargeted metabolomic data. Analytical Chemistry, 84, 5035-5039.
[45] Abaffy, T., Moller, M.G., Riemer, D.D., et al. (2013) Comparative analysis of volatile metabolomics signals from melanoma and benign skin: A pilot study. Metabolomics, 9, 998-1008.
[46] Trygg, J., Holmes, E. and Lundstedt, T. (2007) Chemometrics in metabonomics. Journal of Proteome Research, 6, 469-479.
[47] 阿基业 (2010) 代谢组学数据处理方法—主成分分析. 中国临床药理学与治疗学, 5, 481-489.
[48] Chen, J., Wang, W.Z., Lv, S., et al. (2009) Metabonomics study of liver cancer based on ultra performance liquid chromatography coupled to mass spectrometry with HILIC and RPLC separations. Analytica Chimica Acta, 650, 3-9.
[49] Song, H., Peng, J.S., Yao, D.S., et al. (2012) Serum metabolic profiling of human gastric cancer based on gas chromatography/mass spectrometry. Brazilian Journal of Medical and Biological Research, 45, 78-85.
[50] GO, E.P. (2010) Database resources in metabolomics: An overview. Journal of Neuroimmune Pharmacology, 5, 18-30.
[51] 张良晓, 陈爱明, 梁逸曾, 等 (2008) 人类代谢组学GC-EIMS标准物质数据库的建立. 第二届全国生命分析化学学术报告与研讨会, F12, 292.

为你推荐