红水河鲢线粒体D-Loop和Cyt b基因序列分析

Comparison of Mitochondrial D-Loop and Cyt b Sequences of Hypophthalmichthys molitrix

摘要: 研究了红水河野生鲢Hypophthalmichthys molitrix上游、中游和下游3个群体24个个体的线粒体控制区(D-loop)和细胞色素b (Cyt b)基因序列，通过PCR扩增与测序，分别获得了长度为820 bp和1117 bp的D-loop和Cyt b基因片段。分析表明，D-loop序列共定义了20个单倍型，存在74个多态位点，发生转换19次、颠换3次、插入/缺失52次。Cyt b序列共定义了9个单倍型，存在48个多态性位点，发生转换13次、颠换1次、插入/缺失34次。两序列分析结果表明，群体间和群体内遗传距离较小，遗传变异的分子变异等级不显著。上、中、下游均有共享单倍型，3个群体间遗传分化不大。

Abstract: Sequences of mitochondrial D-loop and Cyt b gene of 24 individuals of Hypophthalmichthys molitrix which were sampled from three populations in Hongshui River were determined. Using PCR amplification and sequencing, 820 bp of D-loop partial sequences and 1117 bp of Cyt b partial se-quences were obtained. In D-loop sequences, a total of 74 polymorphic sites were defined; 20 dis-tinct haplotypes, 19 transitions, 3 transversions and 52 insert/lacuna loci were found. In Cyt b se-quences, 48 polymorphic sites and 9 haplotypes were defined; 13 transitions, 1 transversion and 34 insert/lacuna loci were found. Two-sequence analysis showed that, within groups and between groups, the genetic distance is small, and the genetic variation of molecular variation level is not significant. Upper, middle and lower reaches are shared haplotypes, and the genetic differentiation among three populations is little.