PCDH10基因DNA甲基化在胃癌中的研究

Research of PCDH10 DNA Methylation in Gastric Cancer

摘要: 目的：探讨PCDH10(原钙粘蛋白10)基因DNA发生甲基化后，其表达与胃癌发生的关系。方法：采用甲基化特异性PCR (methy-lation specific PCR)法和实时荧光定量逆转录–多聚合酶链反应(real-time RT-PCR)方法检测100例胃癌组织及正常组织中PCDH10基因启动子区的甲基化情况。结果：胃癌组织中PCDH10基因的甲基化阳性率较正常组织明显升高，差异具有统计学意义。结论：PCDH10的基因DNA甲基化可能与胃癌发生有关。

Abstract: Objective: To analyze and compare methylation status of PCDH10 gene in gastric cancer and nomal tissues. Methods: Methylation-Specific PCR (MSP) and RT-PCR (real-time RT-PCR) were applied to detect DNA methylation features of the PCDH10 gene in 100 smples. Results: The PCDH10 gene methylation in the gastric cancer tissues was significantly higher than that in normal tissues. Con-clusion: PCDH10 DNA methylation is correlated with gastric cancer.