免疫印迹法测定健康体检人群幽门螺旋杆菌抗体及毒性分型结果分析

Using Western Blod Assay to Analyze H. pylori Antibody and Toxicity Typing of Health Check-Up Group

摘要: 目的：本文回顾性分析2012年12月至2014年12月健康体检人群不同年龄段幽门螺旋杆菌感染类型的情况，即感染后产生的抗体及毒性的分布特征。方法：采用免疫印迹法对健康体检人群的血清进行免疫表型检测和分型，以年龄进行分组统计，检测结果采用χ2检验进行分析，P < 0.05表示差异有统计学意义。 结果：17,052体检者II型Hp感染尿素酶A或/和尿素酶B的阳性率为33.27% (5674/17,052)，阳性率最高的年龄分布是30~69岁；I型Hp感染细胞毒素A或/和空泡毒素A的阳性率为10.58% (1804/17,052)，阳性率最高的年龄分布是30~49岁；II型Hp感染及II型Hp感染的阳性率在不同的年龄组之间存在差异(P < 0.05)，低年龄组(18～30岁)和高年龄组(>70岁) Hp感染的阳性率低。结论：在健康体检的人群中，无论是Hp感染率，还是Hp感染后的免疫分型结果，都与临床病例存在一定的差异，Hp的感染及免疫分型在健康体检的人群中存在特殊的分布特征。

Abstract: Objective: To analyze the infection types of Helicobacter pylori in different age groups from December 2014 to December 2012, i.e. antibodies and characteristics of toxicity distribution after infection. Methods: We use Western blotting method to detect and classify the immunophenotype of serum of physical examination population. Age is used as the statistical unit, results are tested and analyzed by χ2, and P < 0.05 means that the difference has statistical sense. Results: The positive rate of type II Hp infection of A urease or/and urease B for the check-up was 33.27% (5674/17,052), and age distribution ranging from 30 to 69 enjoys the highest positive rate; the positive rate of type I Hp infection of cytotoxin A or/and vacuolate cytotoxin A was 10.58% (1804/17,052), and age ranging from 30 - 49 enjoys the highest positive rate. In different age groups of I and II, there exist differences (P < 0.05): low age group (18 - 30 years old) and old age group (>70 years) have lower possibilities to infect Hp. Conclusion: Among physical examination population, the rate of Hp infection and the immune typing results after infection, compared with clinical cases, both have some differences. Hp infection, as well as immune typing, exists special distribution characteristics among physical examination population.