基于分子间分裂式DNA酶和磁纳米颗粒的生物传感器用于Hg2+的比色检测
A Sensitive Colorimetric Biosensor Based on Intermolecular Split G-Quadruplex DNA Zymes and Magnetic Nanoparticles for the Detection of Hg2+
摘要: 本文设计了一种新的比色生物传感器检测Hg2+，主要是基于建立分子间的分裂式DNA酶，利用核酸序列中的胸腺嘧啶(T)能够特异性识别Hg2+，形成特殊的T-Hg-T结构。SA是一段富含T碱基组装到磁纳米颗粒表面寡聚核苷酸的片段(MNPs/SA)。SB和SC是两段分离的核酸序列，一端富含T碱基，用来与Hg2+形成T-Hg-T结构；另一端富含G碱基，用于组装G-四联体DNA酶。当体系中存在Hg2+时，SA、SB和SC上的T碱基与Hg2+结合形成T-Hg-T双链结构，SB和SC就组装到了磁颗粒上(MNPs/SA/SB/SC)，此时SB和SC上两段富含G的片段相互靠近，在hemin的存在下，形成hemin嵌入式G-四分体DNA酶可催化H2O2氧化ABTS使其在421 nm处的特征吸收峰值升高。因此，可以通过比较特征吸收峰值来反映溶液中Hg2+的浓度变化情况，传感器对Hg2+的检出限为0.8 nM。100倍的其他金属离子和10,000倍的Cl-共存时都不干扰Hg2+的检测，说明该传感器对汞离子具有高的灵敏度和特异性。基于此，该比色生物传感器有望应用于饮用水和环境水中Hg2+的监测。
Abstract: In this paper, we report a novel and sensitive optical sensing protocol for the detection of mercury (II) ions based on colorimetry combined with intermolecular split G-quadruplex DNA zymes and the conjugation of thymine-Hg2+-thymine. A T-rich strand A was assembled onto the biomagnetic beads. Two other oligonucleotide strands (strands B and C) were employed, which are each composed of a capture segment (T-rich part) and a sensing segment (G-rich part). In the presence of Hg2+, the capture segments of both strands B and C can hybridise with biotin-strand A on magnetic beads to form the stable DNA duplexes through THg2+-T linkages. The formation of the DNA duplex brings the sensing segments of both strands B strand C close enough to constructed an intermolecular split G-quadruplex. This split G-quadruplex is able to effectively catalyse the H202- mediated oxidation of ABTS in the presence of hemin, generating the blue-green product ABTS+ and increasing the absorption signal at 421 nm. Therefore, the change in the absorption signal can be used to monitor the concentration of Hg2+ with the detection limit of 0.8 nM. The other metal ions is 100 times and chloride ion is 10,000 times of mercury ion have slight effects on the UV-Vis absorption of the sensing system, indicating that the sensing assay described herein exhibits a high specificity for Hg2+. The practical results obtained imply that the proposed colorimetric biosensor can be extended for the detection of trace Hg2+ in environmental water and drinking water.
文章引用：张维, 汤玉娇, 邵爽, 戴诗岩, 程圭芳, 何品刚, 方禹之. 基于分子间分裂式DNA酶和磁纳米颗粒的生物传感器用于Hg2+的比色检测[J]. 分析化学进展, 2017, 7(1): 21-30. https://doi.org/10.12677/AAC.2017.71004

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