密度、光照强度及光周期与卤虫休眠卵孵化率间的关系

Relationship of Density, Light Intensity, Photoperiod to Hatching Rate of Artemia Resting Eggs

摘要: 本试验采用响应面法和中心复合试验设计的方法，考察了卤虫密度(D)、光照强度(L)以及光照周期(P) 与卤虫休眠卵孵化率之间的关系。试验结果表明，密度的一次效应对卤虫孵化的影响显著(P < 0.05)、二次效应对孵化的影响极显著(P < 0.01)，随着密度的增加，孵化率呈先上升后下降的趋势。光照强度的一次效应、二次效应的影响均不显著(P > 0.05)。光周期的一次效应对孵化的影响极显著(P < 0.01)、二次效应对孵化影响显著(P < 0.05)，光周期越长，孵化率越高。密度与光照强度间无交互作用(P > 0.05)，密度与光照周期两者之间的交互作用极显著(P < 0.01)，光照强度与光照周期间无交互作用。密度、光照强度、光照周期对孵化率的二次多元回归方程的拟合程度高，可用来预测，最佳优化条件组合为密度1.12 g∙L−1，光照强度为1598 lux、光照周期为23L:01D，孵化率可达96.56%，可靠性达91.35%。

Abstract: Using response surface methodology and central composite design, we examined the relationships among density (D), light intensity (L), photoperiod (P) and hatching rate of artemia resting eggs. The results showed that the linear effect of density had significant effect on hatchability (P < 0.05), and the quadratic effect of density was very significant (P < 0.01). With the increase of density, the hatching rate decreased after an initial increase. The linear and quadratic effects of light intensity were not significant (P > 0.05). The linear effect of photoperiod was very significant (P < 0.01); the quadratic effect of photoperiod had significant on hatch (P < 0.05). The longer the photoperiod, the higher the hatching rate. No significant intensity interactive effect was found between density and light intensity on hatchability (P > 0.05), and the interaction between light intensity and photoperiod was very significant (P < 0.01); light intensity and photoperiod had no significant interaction on the hatching rate. The quadratic multiple regression equation of hatching rate towards density, light intensity and photoperiod had high goodness of fit, and could be used to predict; the optimum condition for hatch was the combination of density 1.12 g∙L−1, light intensity 1598 lux and photoperiod 23L:01D, under which the hatchability reached 96.56% with the reliability peaked to 91.35%.