流动注射—光纤光谱仪测定石榴皮等植物中的总黄酮

Determination of Total Flavonoids in Pomegranate Peels with a Flow Injection-Fiber Optic Spectrophotometric System

摘要: 自组装流动注射-光纤光谱分析仪，建立测定石榴皮、红茶等植物中总黄酮含量的方法。基于黄酮类物质中的酚羟基与AlCl3生成络合物，在414 nm波长产生特征吸收峰，测定石榴皮、玫瑰花等植物中的黄酮含量，并优化实验条件，适宜条件如下：AlCl₃的体积分数为1.3%，温度为20℃，反应管长度为4 m；以75%乙醇-25%水体系为载流，流速为1 mL/min。单一样品分析时间为2 min；浓度在5~100 mg/L (r = 0.9995)范围内与吸光度呈良好的线性关系；回收率在95.31%~99.90%之间，相对标准偏差(RSD)为4.67%。本法简便快速、重现性好、准确度高，实现了植物中总黄酮类化合物检测的快速自动化分析。

Abstract: A flow injection-fiber optic spectrophotometric system was applied to the determination of total flavonoids in pomegranate peels and other plants. This method was based on the reaction of phe-nolic hydroxyl group with AlCl3, and the product has maximum absorption at wavelength of 414 nm, the content of flavonoids in pomegranate peel, rose and other plants were determined and the optimized reaction conditions were: 1.3% of AlCl₃, at 20˚C, the reaction tube was 4 m, 75% ethanol and 25% water solution as carrier with flow rate of 1 mL/min. Under the optimum experimental conditions, the method enables measurement of a single sample in 2 min, a linear calibration graph was obtained over the range 5 - 100 mg/L with the linearly dependent coefficient r = 0.9995 and RSD was 4.67%, the recovery between 95.31% and 99.90%. The proposed method was found to be simple, sensitive, rapid, reproducible, accurate and automatic method in the determination of the total flavonoids.