诱导子对药用植物毛状根活性次生代谢物生成的影响
Effect of Elicitors on the Biosynthesis of Active Secondary Metabolite in Hairy Roots of Medicinal Plants
DOI: 10.12677/BR.2017.63019, PDF, HTML, XML, 下载: 1,586  浏览: 4,156  科研立项经费支持
作者: 周佳蕾, 方圆, 陈建伟：南京中医药大学药学院，江苏 南京
关键词: 药用植物；毛状根；诱导子；次生代谢物；Medicinal Plant； Hairy Root； Elicitor； Secondary Metabolite
摘要: 目的：探讨诱导子对药用植物毛状根次生代谢产物生成的影响。方法：查阅了国内外近20年来相关文献，归纳分析了不同类型诱导子及其组合诱导子对药用植物毛状根次生代谢产物合成量的变化值。结果：诱导子及其组合诱导子可明显提高16种药用植物毛状根中酚酸类、醌类、黄酮类、萜类、三萜皂苷类、生物碱类等次生代谢产物的合成量。结论：诱导子对药用植物毛状根产生次生代谢产物的合成具有促进作用。
Abstract: Objective: To discuss the changes of secondary metabolites in hairy roots of medicinal plants induced by elicitors. Methods: According to related documents both at home and abroad in recent 20 years, the paper systematically analyzes the effects of different elicitors and co-elicitors on the accumulation of secondary metabolites in hairy roots of medicinal plants. Results: Elicitors and co-elicitors can obviously improve the biosynthesis of phenolic acids, quinones, alkaloids, flavonoids, terpenoids, triterpenoidsaponins in hairy roots of 16 medicinal plants. Conclusion: The production of secondary metabolites in hairy roots of medicinal plants can be increased by elicitors.
文章引用：周佳蕾, 方圆, 陈建伟. 诱导子对药用植物毛状根活性次生代谢物生成的影响[J]. 植物学研究, 2017, 6(3): 139-148. https://doi.org/10.12677/BR.2017.63019

参考文献

[1] Chilton, M.D., Tepfer, D.A., Petit, A., et al. (1982) Agrobacterium rhizogenes Inserts T-DNA into the Genomes of the Host Plant Root Cells. Nature, 295, 432-434.
https://doi.org/10.1038/295432a0
[2] 杜旻, 吴晓俊, 王峥涛, 等. 发根农杆菌Ri质粒及其在植物基因工程中的应用[J]. 药物生物技术, 2005, 12(3): 193-196.
[3] 宁文, 曹日强. 真菌诱导物在植物次生代谢中的调节作用[J]. 植物生理学通讯, 1993, 29(5): 321-324.
[4] 齐凤慧, 詹亚光, 景天忠. 诱导子对植物细胞培养中次生代谢物的调控机制[J]. 天然产物研究与开发, 2008, 20(3): 568-573.
[5] 张囡, 杜丽丽, 王冬, 等. 中药酚酸类成分的研究进展[J]. 中国现代中药, 2006, 8(2): 25-28.
[6] 肖莹. 丹参酚酸类成分生源合成的调控研究[D]: [硕士学位论文]. 上海: 第二军医大学, 2009.
[7] 张顺仓, 刘岩, 沈双, 等. 诱导子对丹参毛状根酚酸类和丹参酮类成分积累的影响[J]. 中国中药杂志, 2011, 36(10): 1269-1273.
[8] Bais, H.P., Walker, T.S., Schweizer, H.P., et al. (2002) Root Specific Elicitation and Antimicrobial Activity of Rosmarinic Acid in Hairy Root Cultures of Ocimum basilicum. Plant Physiology and Biochemistry, 40, 983-995.
[9] 吴立军. 天然药物化学(第6版)[M]. 北京: 人民卫生出版社, 2011: 156.
[10] Monks, T.J., Hanzlik, R.P., Cohen, G.M., et al. (1992) Quinone Chemistry and Toxicity. Toxicology and Applied Pharmacology, 112, 2-16.
[11] 张璞, 王芳, 朱查山. 真菌诱导子与吸附树脂对新疆紫草毛状根中萘醌积累的影响[J]. 生物工程学报, 2013, 29(2): 214-223．
[12] 阎岩, 赵欣, 张顺仓, 等. 活性氧在密旋链霉菌Act12诱导丹参毛状根中丹参酮积累中的作用[J]. 中国中药杂志, 2014, 39(11): 1985-1991.
[13] 裴月湖, 娄红祥. 天然药物化学(第7版)[M]. 北京: 人民卫生出版社, 2016.
[14] 孙际薇, 张鸿, 王凤英, 等. 茉莉酸甲酯对曼陀罗毛状根中主要莨菪烷类生物碱成分积累和释放的影响[J]. 中国中药杂志, 2013, 38(11): 1712-1718.
[15] 段金廒, 陈士林. 中药资源化学(第9版)[M]. 北京: 中国中医药出版社, 2013: 130.
[16] Shinde, A.N., Malpathak, N. and Fulzele, D.P. (2009) Enhanced Production of Phytoestrogenic Isoflavones from Hairy Root Cultures of Psoralea corylifolia L. Using Elicitation and Precursor Feeding. Biotechnology and Bioprocess Engineering, 14, 288-294.
[17] Zhang, H.C., Liu, J.M., Lu, H.Y., et al. (2009) Enhanced Flavonoid Production in Hairy Root Cultures of Glycyrrhiza uralensis Fisch by Combining the Over-Expression of Chalcone Isomerase Gene with the Elicitation Treatment. Plant Cell Reports, 28, 1205-1213.
https://doi.org/10.1007/s00299-009-0721-3
[18] 王剑文, 郑丽屏, 谭仁祥. 促进黄花蒿发根青蒿素合成的内生真菌诱导子的制备[J]. 生物工程学报, 2006, 22(5): 829-834.
[19] Zhang, B., Zou, T., Yan, H., et al. (2010) Stimulation of Artemisinin Biosynthesis in Artemisia annua Hairy Roots by Oligogalacturonides. African Journal of Biotechnology, 9, 3437-3442.
[20] Liby, K.T., Yore, M.M. and Sporn, M.B. (2007) Triterpenoids and Rexinoids as Multifunctional Agents for the Prevention and Treatment of Cancer. Nature Reviews Cancer, 7, 357-369.
https://doi.org/10.1038/nrc2129
[21] Zhou, L.G., Cao, X.D., Zhang, R.F., et al. (2007) Stimulation of Saponin Production in Panax ginseng Hairy Roots by Two Oligosaccarides from Paris polyphylla var. yunnanensis. Biotechnology Letters, 29, 631-634.
https://doi.org/10.1007/s10529-006-9273-6
[22] 徐立新. 植物激素和诱导子对人参发根皂苷含量的影响研究[D]: [硕士学位论文]. 吉林: 吉林大学, 2007.
[23] Wu, J.Y. and Shi, M. (2008) Ultrahigh Diterpenoid Tanshinone Production through Repeated Osmotic Stress and Elicitor Stimulation in Fed-Batch Culture of Salvia miltiorrhizahairy Roots. Applied Microbiology and Biotechnology, 78, 441-448.
https://doi.org/10.1007/s00253-007-1332-y
[24] Jiang, L.Z., Li, G.Z. and Jian, Y.W. (2010) Promotion of Salvia miltiorrhiza Hairy Root Growth and Tanshinone Production by Polysaccharide-Protein Fractions of Plant Growth-Promoting Rhizobacterium Bacillus cereus. Process Biochemistry, 45, 1517.
[25] Yan, Q., Hu, Z.D., Tan, R.X., et al. (2005) Efficient Production and Recovery of Diterpenoid Tanshinones in Salvia miltiorrhiza Hairy Root Cultures with in Situ Adsorption, Elicitation and Semi-Continuous Operation. Journal of Biotechnology, 119, 416-424.
[26] Zhao, J.L., Zhou, L.G. and Wu, J.Y. (2010) Promotion of Salvia miltiorrhiza Hairy Root Growth and Tanshinone Production by Polysaccharide-Protein Fractions of Plant Growth-Promoting Rhizobacterium Bacillus cereus. Process Biochemistry, 45, 1517-1522.
[27] Wu, J.Y., Ng, J., Shi, M., et al. (2007) Enhanced Secondary Metabolite (Tanshinone) Production of Salvia miltiorrhiza Hairy Roots in a Novel Root-Bacteria Co-Culture Process. Applied Microbiology and Biotechnology, 77, 543-550.
https://doi.org/10.1007/s00253-007-1192-5
[28] 王学勇, 崔光红, 黄璐琦, 等. 茉莉酸甲醋对丹参毛状根中丹参酮类成分积累和释放的影响[J]. 中国中药杂志, 2007, 32(4): 300-302.
[29] Pitta-Alvarez, S.I., Spollansky, T.C. and Giulietti, A.M. (2000) The Influence of Different Biotic and Abiotic Elicitors on the Production and Profile of Tropane Alkaloids in Hairy Root Cultures of Brugmansia candida. Enzyme and Microbial Technology, 26, 252-258.
[30] Khelifi, L., Zarouri, B., Amdoun, R., et al. (2011) Effects of Elicitation and Permeabilization on Hyoscyamine Content in Datura stramonium Hairy Roots. Advances in Environmental Biology, 5, 329-334.
[31] Chae, Y.A., Yu, H.S., Song, J.S., et al. (2000) Indigo Production in Hairy Root Cultures of Persicaria tinctoria Lour. Biotechnology Letters, 22, 1527-1530.
https://doi.org/10.1023/A:1005668625822
[32] Thakore, D., Srivastava, A.K. and Sinha, A. (2012) Enhanced Production of Antihypertensive Drug Ajmalicine in Transformed Hairy Root Culture of Catharanthusroseus by Application of Stress Factors in Statistically Optimized Medium. Health, Energy and Environmental Perspectives. In: Srivastava, M.M., Khemani, L.D. and Srivastava, S., Eds., Chemistry of Phytopotentials, Springer, Berlin, 39-42.
[33] Hasanloo, T., Sepehrifar, R., Rahnama, H., et al. (2009) Evaluation of the Yeast-Extract Signaling Pathway Leading to Silymarin Biosynthesis in Milk Thistle Hairy Root Culture. World Journal of Microbiology and Biotechnology, 25, 1901-1909.
https://doi.org/10.1007/s11274-009-0088-1
[34] Liu, C., Wang, Y., Xu, X., et al. (1999) Improvement of Artemisinin Accumulation in Hairy Root Cultures of Artemisia annua L by Fungal Elicitor. Bioprocess and Biosystems Engineering, 20, 161-164.
https://doi.org/10.1007/pl00009041
[35] Putalun, W., Luealon, W., De-Eknamkul, W., et al. (2007) Improvement of Artemisinin Production by Chitosan in Hairy Root Cultures of Artemisia annua L. Biotechnology Letters, 29, 1143-1146.
https://doi.org/10.1007/s10529-007-9368-8
[36] Wielanek, M. and Urbanek, H. (2006) Enhanced Glucotropaeolin Production in Hairy Root Cultures of Tropaeolummajus L. by Combining Elicitation and Precursor Feeding. Plant Cell, Tissue and Organ Culture, 86, 177-186.
https://doi.org/10.1007/s11240-006-9106-2
[37] 齐香君, 郭乐康, 陈微娜. 诱导子对黄芩毛状根生长及黄芩苷合成的影响[J]. 中草药, 2009, 40(5): 801-803.
[38] 朱葵荣, 马琳, 冯杉. 不同诱导子对黄芩不定根生长及黄芩苷累积的影响[J]. 中南药学, 2014, 12(11): 1076-1079.
[39] Zaheer, M., Reddy, V.D. and Giri, C.C. (2016) Enhanced Daidzin Production from Jasmonic and Acetyl Salicylic Acid Elicited Hairy Root Cultures of Psoraleacorylifolia L. (Fabaceae). Natural Product Research, 30, 1542-1547.
https://doi.org/10.1080/14786419.2015.1054823
[40] 王瑜, 杨世海. 不同诱导子对王不留行毛状根生长和王不留行黄酮苷含量的影响[J]. 人参研究, 2014, 2: 51-53.
[41] Zhou, L., Yang, C., Li, J., et al. (2003) Heptasaccharide and Octasaccharide Isolated from Paris polyphylla var. yunnanensis and Their Plant Growth Regulatory Activity. Plant Science, 165, 571-575.
[42] 于丽莉. 诱导子调控下人参发状根皂苷合成相关基因表达的研究[D]: [硕士学位论文]. 吉林: 吉林农业大学, 2014.
[43] 赵鸿莲, 于荣敏. 诱导子在植物细胞培养中的应用研究进展[J]. 沈阳药科大学学报, 2000, 17(2): 152-156.
[44] Radman, R., Saez, T., Bucke, C., et al. (2003) Elicitation of Plants and Microbial Cell Systems. Biotechnology and Applied Biochemistry, 37, 91-102.
https://doi.org/10.1042/ba20020118
[45] Newton, J.A. and Fray, R.G. (2004) Integration of Environmental and Host Derived Signals with Quorum Sensing during Plant-Microbeinter Actions. Cellular Microbiology, 6, 213-224.
https://doi.org/10.1111/j.1462-5822.2004.00362.x
[46] Jian, Z., Davis, L.C. and Verpoorte, R. (2005) Elicitor Signal Transduction Leading to Production of Plant Secondary Metabolites. Biotechnology Advances, 23, 283-333.