祛脂三味方对非酒精性脂肪肝小鼠肝功能和血脂的影响

Effects of Quzhi Sanwei Fang on Liver Function and Serum Lipid in Mice with Nonalcoholic Fatty Liver Disease

摘要: 目的：观察祛脂三味方对非酒精性脂肪肝小鼠肝功能和血脂的影响。方法：C57小鼠随机分为正常对照组8只、模型对照组8只和祛脂三味方组21只，正常对照组给予正常饲料，其余给予高脂饲料14周复制非酒精性脂肪肝模型；祛脂三味方组在造模第一天开始按照16 g/Kg灌胃祛脂三味方，每天一次，共计14周，其余给予同体积生理盐水。实验结束观察各组小鼠体重、肝脏指数、肝脏HE以及血清ALT，AST，TC，TG，HDL，LDL含量的变化。结果：(1) 与正常组相比，模型组体重显著升高(P < 0.01)而肝脏指数显著下降(P < 0.01)，与模型组相比较，祛脂三味方组体重显著下降(P < 0.05)，但肝脏指数变化不明显；(2) 模型组肝脏细胞内可见弥漫性小泡和散在的大泡，肝细胞脂肪变明显；与模型组相比较，祛脂三味方组未见大泡，肝细胞内仅少量小泡可见；(3) 与正常组相比，模型组大鼠ALT和AST显著升高(P < 0.01和P < 0.05)，与模型组相比较，祛脂三味方组ALT和AST则显著降低(P < 0.01, P < 0.01)；(4) 与正常组相比，模型组大鼠血清中TC含量明显升高(P < 0.05)，TG和HDL有一定程度升高，但无统计学差异；LDL稍微下降，但无统计学差异；相比模型组，祛脂三味方组大鼠血清TC含量显著下降(P < 0.05)，TG，HDL和LDL略有下降，但无统计学意义。结论：祛脂三味方预防性给药能减轻非酒精性脂肪肝小鼠肝细胞脂肪变，具有显著的保肝降酶和降血脂作用。

Abstract: Objective: To investigate the effect of Quzhi sanwei fang on liver function and serum lipid in mice with nonalcoholic fatty liver disease (NAFLD). Method: C57 mice were randomly divided into normal control group (NC, n = 8), model control group (MD, n = 8) and Quzhi sanwei fang group (QZSWF, n = 21), the NC group was fed with a normal diet, while the others were fed with high fat diet for 14 weeks to duplicate the model of NAFLD. The QZSWF group was fed QZSWF at the dose of 16 g/Kg/d on the first day of modeling, the rest were given the same volume of saline, and all treatments were kept 14 weeks. The changes of body weight, liver index, liver HE stain, and serum concentrations of ALT, AST, TC, TG, HDL and LDL were observed at the end of the experiment. Results: (1) Compared with the NC group, the body weight of the MD group increased significantly (P < 0.01) while liver index decreased significantly (P < 0.01), compared with the MD group, QZSWF group significantly decreased (P < 0.05), but the liver index did not change significantly. (2) In the model group, diffuse microvesicular steatosis, a few macrovesicular steatosis and significant steatosis of hepatocytes were found in liver cells, while only a few microvesicular steatosis exist in the hepatocytes of QZSWF group. (3) Compared with the NC group, the serum concentrations of ALT and AST of MD group rats were significantly higher (P < 0.01 and P < 0.05), but the QZSWF group rats were significantly lower than MD group rats (P < 0.01, P < 0.01). (4) Compared with the NC group, the serum content of TC in MD group rats was significantly increased (P < 0.05), while QZSWF group decreased significantly compared with the MD group (P < 0.05). Conclusion: QZSWF effectively alleviates steatosis of NAFLD mouse hepatocytes prophylactically, also with significant effects on protecting hepatocytes, lowering transaminase and reducing blood fat.