中草药对结核分枝杆菌生物膜的干预

Intervention of Chinese Herbal Medicine on Biofilm of Mycobacterium Tuberculosis

摘要: 结核病(TB)是由结核分枝杆菌(MTB)引起的传染性疾病，治疗时间长是结核防控面临的主要问题。结核杆菌能形成生物膜后，对抗结核药物的敏感性下降，因此，干预结核杆菌生物膜是治疗结核的新思路。本试验随机选择了11种中草药，评价其对结核杆菌生物膜的干预作用，结果表明，苦参(0.4 mg/ml)能抑制结核杆菌生物膜的形成，山茱萸(0.4、0.8、1.6、3.2、6.4 mg/ml)能促进结核杆菌生物膜的形成,并呈明显的量效关系。研究结果提示，不同中草药对结核杆菌生物膜的形成存在不同的干预结果。进一步开展活性跟踪研究，将为发现干预结核杆菌生物膜的分子骨架奠定基础。

Abstract: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB). Long treatment time is the main problem for TB prevention and control. Mycobacterium tuberculosis can form biofilm, which could decrease bactericidal activity of anti-tuberculosis drugs. Therefore, the intervention of Mycobacterium tuberculosis biofilm is a new idea for the treatment of tuberculosis. Eleven Chinese herbs were randomly selected to evaluate their interventional effect on the formation of Mycobacterium tuberculosis biofilm. The results showed that Sophora flavescens (0.4 mg/ml) could inhibit the formation of Mycobacterium tuberculosis biofilm, and Cornus officinalis (0.4, 0.8, 1.6, 3.2, 6.4 mg/ml) could promote the formation of Mycobacterium tuberculosis biofilm and showed a dose-effect relationship. The results showed that different Chinese herbal medicine had different effect on the formation of Mycobacterium tuberculosis biofilm. Further study by bioassay guided isolation will lay the foundation for the discovery of the molecular skeleton with the interventional effect on formation of Mycobacterium tuberculosis biofilm.