不同贮藏条件对潍县萝卜膜脂过氧化作用的影响

Effect of Storage Conditions on Membrane Lipid Peroxidation in Weixian Radish

摘要: 研究不同贮藏条件下萝卜体内膜脂过氧化有关酶活性及膜质过氧化产物含量的变化对于萝卜的贮藏保鲜具有重要意义。本论文通过比色法测定过氧化物酶(POD)、滴定法测定过氧化氢酶(CAT)、氮蓝四唑法测定超氧化物歧化酶(SOD)活性及硫代巴比妥酸法测定丙二醛(MDA)含量，从而研究不同贮藏条件对其膜脂过氧化作用的影响。结果表明：随着贮藏时间的延长，萝卜体内POD、CAT和SOD的活性总体呈下降趋势，而且不同贮藏条件下萝卜体内三种酶活性降低幅度也不一样，其中低温保鲜贮藏条件下萝卜体内活性下降最慢，常温条件下下降最快；丙二醛的含量则随着贮藏时间的延长含量逐渐上升，其中常温条件下的萝卜体内丙二醛含量上升最快，低温保鲜贮藏条件下的萝卜体内含量上升最慢。

Abstract: It is important to study the activities of enzyme and the content of product related to membrane lipid peroxidation for storage and preserving fresh in Weixian Radish. In the experiment the activities of pe-roxidase (POD), Catalase (CAT), superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were measured, and the effect of storage conditions was studied. The results were as below. The activities of POD, CAT and SOD decreased generally in Weixian Radish with the storage time delayed, but there were difference among treatments, the activities of enzymes in low temperature plus preserving fresh condition decreased most slowly, the activities of them in normal conditions deceased most quickly. The content of MDA increased gradually with the storage time delayed. In normal condition the content of MDA increased most quickly, and in low temperature plus preserving fresh condition increased most slowly.