Ca2+信号通路介导白藜芦醇诱导SGC-7901肿瘤细胞凋亡的研究

Effect Mechanism of Resveratrol Induces SGC-7901 Apoptosis via Ca2+ Signal Pathway

摘要: 目的：研究白藜芦醇(Res)诱导SGC-7901肿瘤细胞凋亡的Ca2+信号通路的机制。方法：MTT法测定Res对SGC-7901细胞抑制率；流式细胞仪检测Res对肿瘤细胞凋亡和肿瘤细胞周期的影响；荧光显微镜从形态学鉴定肿瘤细胞凋亡。激光共聚焦法测定白藜芦醇对肿瘤细胞Ca2+活性的影响。结果：Res明显抑制肿瘤细胞的增值，且呈一定剂量依赖关系，其IC50为164.69 μmol?L–1，当Res剂量为44、88、176 μmol?L–1时，肿瘤细胞的抑制率为：29.693%，33.986%，85.634%；此外，肿瘤细胞周期G1期细胞的比例减少，S期细胞的比例增加，G2/M期减小；通过形态学观察发现，随着Res剂量的增加，镜下凋亡细胞比例逐渐增加，肿瘤细胞的核浆比减小，细胞核高度固缩，染色程度加深，凋亡小体的数量不断增加。其对Ca2+含量的影响分别为18.0463 ± 1.0798、19.7550 ± 2.1129和26.6300 ± 2.0813。结论：Res明显抑制SGC-7901细胞的增殖且诱导其凋亡并影响Ca2+信号通路。

Abstract: Objective: To study effect of resveratrol (Res) induce SGC-7901 cell apoptosis via Ca2+ signal. Methods: Use the MTT method to test the inhibition rates of Res in SGC-7901 tumor cells; Use FCM to detect the influence of the Res on tumor cell apoptosis and cell cycle; Use fluorescence microscope to identify the tumor cell apoptosis in the morphology. Results: Res inhibit SGC-7901 cell proliferatrion in a dose- dependent manner, Its IC50 is 164.69 μmol?L–1. When the concentration of Res is 44, 88, 176 μmol?L–1, the rates of proliferatrion are 29.693%, 33.986%, 85.634%. G1 term is gradually diminished, S term become gradually increasement. G2/M term become gradually reduction. According to the morphology, the apoptosis body become increase, tumor cell’s caryoplasm become reduction and tumor cell karyopyknosis, the extent of the colour become deeply with the concentration of the Res. content of Ca2+ are 18.046 ± 1.0798; 19.7550 ± 2.1129; 26.6300 ± 2.0813 respectively. Conclusion: Res can inhibit the tumor cell prolieratrion and induce the tumor cell apoptosis via Ca2+ sigal pathway