基于核壳式量子点CdSe-PDT光漂白规律的实验研究
Experimental Research on the Photobleaching Mechanism of PDT Based QDs-CdSe
摘要: 近年来随着光动力疗法基础研究的不断深入，光敏剂光漂白及其机制研究日渐成为PDT研究的热点问题。本实验利用化学反应动力学原理对量子点CdSe在胎牛血清(FBS)和HL60细胞悬液的复杂溶液体系中的光漂白速率进行理论推导，得出其光漂白在单一溶液里符合一级动力学过程，在复杂溶液中满足二级反应动力学过程的结果。并通过测定量子点CdSe的紫外可见吸收光谱，利用比尔–朗伯吸收定律得到量子点CdSe的光漂白曲线和光漂白速率，通过拟合比较得出光漂白反应动力学规律，对实验结果进行了理论验证。
Abstract: With the development of the basic research of photodynamic therapy (PDT), photosensitizer photobleaching has become a highly focused area of research within PDT in recent years. In this paper, the photobleaching mechanism of quantum dots in the FBS solution and HL60 cell solutions with oxygen substrate were investigated by the method of theoretical derivation. The results demonstrated that the photobleaching in the single solution was corresponded with the first order kinetics while the photobleaching in the complex solution was corresponded with the second order kinetics. The curve and rate of CdSe photobleaching were obtained by determining the vis absorption spectrum of the CdSe and using of the Beer. Additionally, the photobleaching properties of quantum dot (CdSe) whose theoretical derivation can be confirmed by our experiment were gained.
文章引用：王健, 胡冬香, 邓解关, 黄康强, 熊建文. 基于核壳式量子点CdSe-PDT光漂白规律的实验研究[J]. 生物医学, 2012, 2(1): 6-10. http://dx.doi.org/10.12677/hjbm.2012.21002

参考文献

[1] S. Hackbarth, J. Schlothauer, A. Preu, et al. New insights to pri- mary photodynamic effects—singlet oxygen kinetics in living cells. Journal of Photochemistry and Photobiology B: Biology, 2009, 3(98): 173-179.
[2] D. Sutoa, Y. Iuchia, Y. Ikedab, et al. Inactivation of cysteine and serine proteases by singlet oxygen. Archives of Biochemistry and Biophysics, 2007, 2 (461): 151-158.
[3] S. Jensena, C. Rolinb and R. Ipsena. Stabilisation of acidified skimmed milk with HM pectin. Food Hydrocolloids, 2010, 4(24): 291-299.
[4] C. L. Yan, X. W. Fu, et al. Mitochondrial behaviors in the vitrified mouse oocyte and its parthenogenetic embryo: Effect of taxol pretreatment and relationship to competence. Fertility and Sterility, 2010, 3(93): 959-966.
[5] 顾瑛, 刘凡光. 光动力疗法[M]. 北京: 人民卫生出版社, 2004: 10-11.
[6] N. Breusing, S. Grimm, D. Mvondo, et al. Light-induced cyto- toxicity after aminolevulinic acid is mediated by heme and not by iron. Journal of Photochemistry and Photobiology B: Biology, 2010, 1(99): 36-43.
[7] B. C. Wilson, M. S. Patterson and L. Lilge. Implicit and explicit dosimetry in photodynamic therapy: A new paradigm. Lasers in Medical Science, 1997, 3(12): 182-199.
[8] R. Generalov, S. Kavaliauskiene1, S. Westrom, et al. Entrapment in phospholipid vesicles quenches photoactivity of quantum dots. International Journal of Nanomedicine, 2011, 6(2011): 1875-1888.
[9] 尤小刚, 贺蓉, 高峰等. 核/壳型CdTe@SiO2荧光纳米复合粒子的制备与表征[J]. 化学学报, 2007, 6(65): 561-565.
[10] L. Jasmina, J. C. Sung, M. Francoise, et al. Unmodified cadmium telluride quantum dots induce resctive oxygen species formation leading to multiple organelle damage and cell death. Chemistry and Biology, 2005, 11(12): 1227-1234.
[11] W. C. W. Chan, S. Nie. Quantum Dot Bioconjugates for Ultrasen- sitive Nonisotopic Detection. 1998, 5385(281): 2016-2018.
[12] A. C. Samia, X. B. Chen and C. Burda. Semiconductor quantum dots for photodynamic therapy. Journal of the American Chemi- cal Society, 2003, 51(125): 15736-15737.
[13] W. Yu, L. H. Qu, Z. G. Wen, et al. Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals. Chemistry of Materials. 2003, 14(15): 2854-2860.
[14] Y. Han, G. Y. Xie, N. Liu, et al. Effects of CdTe quantum dots on the antioxidant enzymes activity and lipid peroxidation in testes of mice. Asian Journal of Ecotoxicology, 2010, 6(5): 894-898.
[15] 王颖, 刘凡光, 顾瑛等. 血卟啉单甲醚在不同溶液体系中的光漂白途径研究[J]. 中国激光医学杂志, 2005, 4(14): 268.
[16] 黄乃艳, 顾瑛, 刘凡光等. 卟啉类光敏剂漂白的动力学规律[J]. 中国激光医学杂志, 2005, 1(14): 31-34.
[17] W. H. Chan, N. H. Shiao and P. Z. Lu. CdSe quantum dots induce apoptosis in human neuroblastoma cells via mitochondrial-depen- dent pathways and inhibition of survival signals. Toxicology Letters, 2006, 3(167): 191-200.
[18] 张宝琴, 肖谧, 张镇西等. ALA-PDT破坏K562和HL60白血病细胞实验条件选择的研究[J]. 激光杂志, 2005, 26(2): 85-87.

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