不同产地和不同生长期白芍质量研究

Study on Quality of Radix Paeoniae Alba of Different Origin and Different Growth Stages

摘要: 采用HPLC法测定不同白芍药材中芍药苷含量，确定哪种白芍含芍药苷最高。方法：采用色谱柱：Hypersil C18柱(4.6 × 200 mm，5 μm)；流动相：乙腈/0.05%磷酸水(14:86)；检测：UV 230 nm；流速：1.0 ml/min；柱温：25℃。结果表明：2年生、10月份采收贮存期短的亳白芍芍药苷含量最高。该方法简便，准确可靠，重复性好。

Abstract:  To compare the quality on different Radix Paeoniae Alba using HPLC methods. Column: Hypersil C18 column (4.6 × 200 mm, 5 μm); mobile phase: acetonitrile-H₂O (acidified to 0.05% with phosphoric acid) (14:86); UV test: 230 nm; flow-rate: 1.0 ml/min; column temperature: 25˚C. There was the highest content of paeoniflorin in Radix Paeoniae Alba of two-year-growing time, harvest time in October and short storage time from Bozhou. The assay methods are simple, rapid and with good reproducibility.