内生细菌YBS106菌株发酵条件优化

Optimization of Fermentation Conditions for Endophytic Bacteria Strains YBS106

摘要: 对一把伞南星内生细菌YBS106发酵条件进行研究，确定其最佳发酵液培养基配方和最佳发酵条件。通过杯碟法测定YBS106发酵液对棉花枯萎病原菌的抑菌作用，研究了该菌株在不同培养基，不同生长条件下的代谢产物活性。菌株YBS106的最佳培养基为：葡萄糖60g/L，硫酸铵2.5g/L，麸皮25g/L，柠檬酸三钠2.0g/L K₂HPO₄·3H₂O 0.2g/L，FeSO₄·7H₂O 0.05g/L，MgSO₄·7H₂O 1.0 g/L。最佳生长条件为：pH7.0，28℃，发酵72 h，接种量4%，揺床转速为200r/min。在最佳培养条件下，该菌株对棉花枯萎病菌的抑菌率可达56%。 To optimize the culture medium and the fermentation conditions, experiment was performed to the fermentation conditions of endophytic bacterium YBS106 isolated from Arisaema erubescens in Emei Mount. The antifungal activity of YBS106 fermenting borth was determined against Fusarium oxysporum with Cylinder plate method, and studied on the activity of metabolic products of strains under different culture medium and growth condition. Results showed that the optimal medium compositions were (g/1000mL): glucose 60 g, ammonium sulfate2.5 g, bran25 g, Trisodium citrate2.0 g, K₂HPO₄·3H₂O 0.2 g, FeSO₄·7H₂O 0.05 g, MgSO₄·7H₂O 1.0 g. The optimal growth conditions were: pH 6.0, 28˚C, 4% inoculation, fermentation time for 72 h with 200 r/min rotating speed. The inhibitory ratio of YBS106 fermenting solution against Fusarium oxysporum could reach to 56% under optimum fermenting solution and culture conditions.