摘要: 本论文以布朗葡萄藻(Botryococcus braunii)357株为材料，研究了7种光质白光、红光、蓝光、混光1(红:蓝 = 4:1)、混光2(红:蓝 = 2:1)、绿光及黄光对藻细胞生长和胞内几种有机物质含量的影响。结果表明：在7种光质培养下，细胞密度和生物量在蓝光下最高，OD680和干重分别可达到1.31和2.56 g∙L−1，高于其他光质；多糖含量在白光下最高，其含量达到0.94%；可溶性蛋白含量在红光最高，为0.51%；叶绿素和类胡萝卜素的含量则在混光1下最高，分别达到1.84%和1.07%；在混光1下的总脂和总烃含量最高，分别为25.02%、25.10%，高于其他光质。这些结果将为布朗葡萄藻光生物反应器的开发，及其生长调控与大规模培养提供依据。
Abstract:
In this study, the influence of different light qualities on growth and organics contents of Botryococcus braunii 357 were investigated. Seven light qualities including white, red, blue, mixed light 1 (red:blue = 4:1), mixed light 2 (red:blue = 2:1), green and yellow were used. The results showed that the cell density and biomass of B. braunii 357 were higher under blue light than the other lights, which were 1.31 (OD680) and 2.56 g∙L−1, respectively. The highest polysaccharide content was observed under white light, reaching to 0.94%; the highest soluble protein was shown under red light, reaching to 0.51%. The content of Chlorophyll and carotenoids under mixed light1 was up to 1.84% and 1.07%, which were significantly higher than other light. Total lipids and total hydrocarbons of B. braunii 357 under mixed light 1 were 25.02% and 25.10% respectively, which were significantly higher than the other light quality. These results will facilitate photobioreactor’s development, growth regulation and large scale cultivation of B. braunii in the future.