高效液相色谱–质谱联用技术在杂环化合物分析中的应用

The Application of High Performance Liquid Chromatography-Mass Spectrometry Analysis of Heterocyclic Compounds

摘要: 高效液相色谱–质谱联用(LC-MS)技术的应用领域极为广阔，化工生产中许多有机物可以用液相色谱法来进行分析，而质谱能够提供物质的结构信息，应用于许多新类型化合物的分析。本文首先介绍了LC-MS联用的基本原理以及发展情况；之后针对原料药化工生产中的检测和遇到的问题介绍了杂环类有机物的定性研究，主要针对保留时间的控制和拖尾因子的改善方法作出讨论。具体研究内容及成果如下：1) 提高大极性化合物的色谱保留性；2) 色谱峰拖尾因子的改善；3) 色谱分离方法的研究。

Abstract: High performance liquid chromatography-mass spectrometry (LC-MS) technology is widely applied in many areas. Chemical production of many organic compounds can be analyzed by liquid chromatography and mass spec- trometry can provide structural information of the substance. LC-MS is used in many new types of compounds analysis. This paper first introduces the basic principles and the development of LC-MS; then heterocyclic organic qualitative re- search of the API for the detection of chemical production and the problems encountered are introduced, and mainly discusses retention time control and trailing factors improvement. Specific studies and the results are as follows: 1) Im-prove chromatographic retention of polar compounds; 2) Peak tailing factor improvement; 3) Study of the chroma-tographic separation methods.