油菜对Sr胁迫的生理生态响应

Physiological and Ecological Responses of Rape Seedlings to Strontium Stress

摘要: 在砂培条件下，采用不同浓度的Sr2+ (0、05、1、5、10 mmol/L)对转pprI基因油菜幼苗(Brassica napus L.)和非转基因油菜幼苗处理30 d，分析油菜幼苗对Sr的吸收积累情况，并研究油菜对Sr胁迫的生理生态响应。结果表明：转基因油菜幼苗和非转基因油菜幼苗对Sr2+的吸收量均随Sr2+胁迫浓度的升高而升高，转基因油菜根部的Sr2+含量大于非转基因油菜根部，而地上部分的Sr2+含量却小于非转基因油菜。与对照相比，在Sr2+胁迫下的转基因油菜和非转基因油菜脯氨酸(Pro)含量则随Sr2+浓度的提高而升高，而SOD、POD活性和叶绿素含量都随Sr2+浓度的增加呈先上升后下降趋势。与非转基因油菜相比，转基因油菜在各种胁迫浓度下的SOD、POD活性、叶绿素含量以及脯氨酸含量都高于非转基因油菜。因此得出结论：转pprI基因油菜较非转基因油菜更具有对Sr胁迫的抗性和耐受能力。

Abstract: PprI-transgenic and non-transgenic rape seedlings (Brassica napus L.) were treated with different concentrations of Sr2+ (0, 0.5, 1, 5, 10 mmol/L) for 30 days under quartz sand culture. The Sr2+ uptake and physiological and ecological characteristics of rape seedlings were investigated. The results showed that the uptake of Sr2+ in transgenic and non-transgenic rape seedlings increased with the increase of Sr2+ stress concentration; the content of Sr2+ in transgenic rape roots was greater than that in non-transgenic, but the content in up-ground parts in transgenic rape was less than that in non-transgenic rape. Contrasted to the CK, the content of Pro increased with the increase of Sr2+ concentration, but the activity of SOD and POD, and the content of chlorophyll first increased then declined. Under the same treatment, the activity of antioxidant enzymes (SOD and POD) and the content of chlorophyll and Pro in transgenic rape were higher than those in non-transgenic rape. So we conclude that: PprI-transgenic rape has better ability of resistance and tolerance to strontium-stress.