葡萄花药培养诱导不出花粉单倍体植株
Pollen Haploid Plants Cannot Be Induced from Grape Anther Culture
DOI: 10.12677/WJF.2014.33005, PDF, HTML,  被引量 下载: 3,402  浏览: 11,684 
作者: 曹孜义:甘肃农业大学生命科学技术学院,兰州;甘肃省老教授协会农业委员会,兰州
关键词: 植物单倍体育种葡萄花药培养单倍体二倍体Plant Haploid Breeding Grape Anther Culture Haploid Diploid
摘要: 本文根据有关文献和自己经历,作者对葡萄单倍体植株诱导培养问题和学术界观点进行了梳理和讨论。对从葡萄花药培养诱导出植株的唯一的一篇论文,从六个方面进行了质疑;依据细胞学、植物形态学、遗传学等方面理论,认为声称得到葡萄单倍体植株仍是二倍体植株。本文指出,根据目前的技术,葡萄花药培养诱导不出花粉单倍体植株;同时,也对果树花药培养诱导单倍体植株和倍性鉴定问题进行了讨论。
Abstract: Based on the literature and his own research experiences, the author presented and discussed the academic point on induction and culturing of grape pollen haploid plants. Upon the only one paper reporting that whole grape plants were induced from anther culture, the author listed six evidences, including cytology, plant morphology and genetics, to clarify that the so-called haploid plants were still diploid plants. Finally, the author proposed that the grape anther culture cannot induce the pollen haploid plants according to the existing technology. At the same time, the issue about the induction of pollen haploid plants from anther culture and ploidy identification in fruit trees was discussed.
文章引用:曹孜义. 葡萄花药培养诱导不出花粉单倍体植株[J]. 林业世界, 2014, 3(3): 23-27. http://dx.doi.org/10.12677/WJF.2014.33005

参考文献

[1] 王延玲, 丰震, 赵兰勇 (2006) 植物花药培养研究进展. 山东农业大学学报(自然科学版), 1, 149-151.
[2] 沈德绪 (1995) 果树育种学(第二版). 农业出版社, 北京, 135-138.
[3] 张绿萍, 陈红 (2007) 园艺植物花药培养研究进展. 安徽农业科学, 17, 5140-5142.
[4] 张圣仓, 魏安智, 杨途熙 (2011) 果树单倍体和加倍单倍体(DH)技术研究与应用进展. 果树学报, 5, 869-874.
[5] 张洁, 张学英, 葛会波 (2002) 果树花药培养研究概况. 河北农业大学学报, 25(增刊), 95-97.
[6] 邹昌杰, 李佩芬 (1981) 葡萄花粉植株的诱导. 植物学报, 4, 79-81.
[7] 邹昌杰, 李佩芬的私人通讯.
[8] 邹昌杰, 等 (1977) 葡萄花药愈伤组织的诱导. 葡萄花药培养学术讨论会文集, 科学出版社, 206-208
[9] 曹孜义, 郭采月, 齐与抠, 等 (1980) 葡萄花药植株诱导成功. 甘肃农业科技, 4, 26-29.
[10] 鲍雪珍, 张建琪, 于洪浩, 邹昌杰, 李佩芬 (1980) 葡萄胚状体发生的初步研究. 山东大学学报(自然科学版), 3, 101-106.
[11] 曹孜义 (1994) 三个生态地区“胜利”葡萄花药植株的倍性. 植物学报, 36(增刊), 151-154.
[12] Mullins, M.G. (1990) Application of tissue culture to the genetic improvement of grapevines. Proceeding of the 5th International Symposium on Grape Breeding Vitis (Special Issue), 399-497
[13] Monette, P.I. (1988) Grapevine (Vitis vinefera L.), biotechnogy in agriculture and forestry. Vol. 6, Springer-Verlag, Berlin, 2-37.
[14] 李佩芬, 卢炳芝, 于向荣, 等 (1995) 葡萄细胞诱变植株的培育. 葡萄栽培与葡萄酿酒, 1, 20-22.
[15] 杨宁, 陈伯鸿, 曹孜义, 郝燕 (2004) 胜利葡萄花药植株结实特性及其后代分离的观察. 河北林业科技, 24, 5658.
[16] 费开韦, 薛光荣 (1981) 元帅苹果花药培养诱导单倍体植株. 中国农业科学, 4, 41-44.
[17] 吴绛云 (1981) 苹果花药培养获得单倍体植株. 园艺学报, 4, 36-37.
[18] 薛光荣, 牛健哲 (1983) 苹果花药培养又获进展. 中国果树, 1, 46.
[19] 薛光荣, 牛健哲 (1984) 诱导苹果花粉植株的研究. 园艺学报, 3, 162-0164.
[20] 薛光荣, 等 (1990) 苹果花药培养技术及8个主栽品种的花粉植株培育成功. 中国农业科学, 3, 86.
[21] 杨振英, 薛光荣, 苏佳明, 迟福梅, 牛健哲, 丛佩华 (2005) 富士花药培养选育出苹果新品种华富. 园艺学报, 1, 172.
[22] 张利义, 杨振英, 丛佩华, 张开春, 刘建利, 薛光荣 (2007) 苹果花药培养植株倍性及其纯合基因型的鉴定. 园艺学报, 2, 481-484.
[23] 新关宏夫 (1976) 花粉育种. 自然(日文), 31, 38-47.
[24] Stamp, J.A. and Meredith, C.P. (1988) Somatic embryogenesis from leaves and amters of grapevine. Sienta Horticultires, 35, 235-250.