大理百合的组织培养研究

Research on Tissue Culture of Lilium taliense Franch

摘要: 采用离体组织培养的方法对大理百合的快速繁殖进行了研究，结果表明：以大理百合的鳞片为外植体进行离体培养，可获得再生植株，并初步建立了其快速繁殖体系。由试验结果可知，适宜大理百合鳞片诱导不定芽和愈伤组织的培养基为MS + 2.0 mg/L6-BA + 0.5 mg/L NAA + 3%蔗糖；而MS + 0.5 mg/L 6-BA + 0.1 mg/L NAA + 3%蔗糖更有利于不定芽的诱导；不定芽增殖的培养基以MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA + 3%蔗糖效果较好；不定芽形成小鳞茎的培养基以MS + 1.0 mg/LNAA + 3%蔗糖效果较好；小鳞茎膨大的培养基以1/2MS + 0.3 mg/L NAA + 0.1 mg/L IBA + 12%蔗糖效果较好。

Abstract: Rapid propagation protocol of Lilium taliense Franch. by in vitro tissue culture was investigated. The results indicated that plant regeneration can be obtained from the bulb scales of L. taliense and a rapid propagation system was established efficiently. Among the media investigated, MS + 2.0 mg/L6-BA + 0.5 mg/LNAA + 3% sucrose is suitable for induction of adventitious bud and callus while MS + 0.5 mg/L6-BA + 0.1 mg/LNAA + 3% sucrose is better for induction of adventitious buds. The medium MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA + 3% sucrose is proper for adventitious bud multiplication; MS + 1.0 mg/L NAA + 3% sucrose is optimum for bulblet formation from the adven-titious buds, while 1/2MS + NAA 0.3 mg/L + IBA0.1 mg/L + 12% sucrose is suitable for the growth of bulblets.