艾叶药材HPLC指纹图谱的鉴别研究

Identification of Argy Wormwood Leaf by HPLC Fingerprint

摘要: 本文的目的是建立艾叶的HPLC指纹图谱鉴别方法，评价艾叶及其混淆品HPLC指纹图谱的相似性。采用RP-HPLC法，以Uitimate-C18 (250 mm × 4.6 mm, 5 μm)色谱柱为分析柱，流动相为乙腈-0.05%磷酸水，梯度洗脱，流速0.60 mL/min，290 nm波长下测定艾叶及其混淆品指纹图谱，并作相似度比较分析。结果建立了艾叶药材HPLC指纹图谱共有模式，有43个共有指纹峰被标定，且相似度较高，艾叶与其混淆品药材的HPLC指纹图谱有明显差异。实验结果表明HPLC指纹图谱具有方法简便，重复性好，特征性强的特点，可用于艾叶药材鉴别。

Abstract: The purpose of this study was to establish HPLC fingerprint method for identification of argy wormwood leaf. Adopting RP-HPLC method, a uitimate-C18 (250 mm × 4.6 mm, 5 μm) column as the analytical column with mobile phase consisting of acetonitrile-0.05% phosphoric acid water, and gradient elution, under the flow rate of 0.60 mL/min at the wavelength of 290 nm, fingerprints of argy wormwood leaf and its adulterant were established, and then similarity comparison analysis was done. Finally we established the HPLC fingerprint’s common pattern of argy wormwood leaf, and 43 common peaks were calibrated, whose similarity was high. There was a significant difference of HPLC fingerprint between argy wormwood leaf and its adulterant. The results showed that HPLC fingerprint was simple and convenient, with good repeatability and strong characteristics, which could be used to identify argy wormwood leaf medicine.