马铃薯脱毒试管苗壮苗技术

Tube Seedling of Virus-Free Robust Technology of Potato

摘要: 以改良1/2 MS为基础培养基，在马铃薯脱毒试管苗组培快繁不同阶段加入不同剂量的植物生长延缓剂B9，配合较低温度和强光条件培养，能够显著改变试管苗的生理指标。试验表明：在23℃和2200 lx以上光照下，马铃薯快繁阶段添加(4~8 mg∙L⁻¹)的B9，有效促进脱毒试管苗茎径、有效茎节数及根数的增加，并且叶片增厚、叶色浓绿，继代周期减到14~18 d，在脱毒苗移栽前的培养基内加入(10~15 mg∙L⁻¹)的B9，21℃和3000 lx下培养，脱毒试管苗其茎变得更粗壮、节间较短、茎木质化程度更高，移栽成活率达97%以上。

Abstract: Based on the modified 1/2 MS medium, different doses of plant growth retardants B9 are added in different stages in rapid propagation of virus-free potato plantlets with lower temperature and light condition, which can significantly change the physiological indices of plantlets. Tests show that at 23˚C and above 2200 lx light, B9 (4 - 8 mg∙L⁻¹) added in potato rapid propagation phase effectively promotes the increase of virus-free plantlets in stem diameter, the number of stem and root, the thickening of leaves, dark green leaves, and successive cycles down to 14 - 18 d. If B9 (10 - 15 mg∙L⁻¹) is added in the medium for virus-free seedlings before transplanting at 21˚C and 3000 lx light, the virus-free plantlets have more robust stems, relatively shorter internodes and higher degree of stem wood; the survival rate reaches 97% per cent.