药用植物茅苍术资源的开发利用现状
Development and Utilization of Medicinal Plant Atractylodes lancea
DOI: 10.12677/BR.2016.53011, PDF, HTML, XML, 下载: 2,383  浏览: 6,403  国家自然科学基金支持
作者: 赵丹, 周佳宇, 戴传超*：南京师范大学生命科学学院，江苏省微生物与功能基因组学重点实验室，江苏省微生物资源产业化工程技术研究中心，江苏 南京
关键词: 茅苍术；道地药材；组织培养；基因工程；微生物工程；Atractylodes lancea； Genuine Medicine； Tissue Culture； Genetic Engineering； Microbial Engineering
摘要: 药用植物茅苍术是我国著名的道地药材。由于近年来对茅苍术的肆意挖掘，导致茅苍术产量锐减，因此亟待保护。本文将综述茅苍术资源现状及人工栽培存在问题，总结生物技术手段在茅苍术药材生产中的应用，为茅苍术的开发利用和保护提供参考。
Abstract: Atractylodes lancea is a famous Chinese genuine medicine used for thousands of years. Because of over exploitation, the storage amount of Atractylodes lancea reduced sharply in recent years. Therefore, A. lancea needs urgent protection. This paper reviews the existing resources of A. lancea and the problems in the medicinal cultivation, and summarizes the biotechnology tools that have been used in the production of A. lancea, aiming at providing reference for the development, utilization and protection of A. lancea.
文章引用：赵丹, 周佳宇, 戴传超. 药用植物茅苍术资源的开发利用现状[J]. 植物学研究, 2016, 5(3): 74-82. http://dx.doi.org/10.12677/BR.2016.53011

参考文献

[1] Li, W.L., Zheng, H.C., Bukuru, J. and De Kimpe, N. (2004) Natural Medicines Used in the Traditional Chinese Medical System for Therapy of Diabetes Mellitus. Journal of Ethnopharmacology, 92, 1-21.
http://dx.doi.org/10.1016/j.jep.2003.12.031
[2] 傅立, 陈潭, 郎楷永, 等. 中国高等植物[M]. 青岛: 青岛出版社, 2001.
[3] 胡世林, 冯学锋, 王玠, 格小光. 中国苍术属——新亚种[J]. 植物分类学报, 2001(1): 84-86.
[4] 郭兰萍, 刘俊英, 吉力, 黄璐琦. 茅苍术道地药材的挥发油组成特征分析[J]. 中国中药杂志, 2002(27): 814-819.
[5] 任冰如, 贺善安. 用RAPD技术评估苍术居群间的亲缘关系[J]. 中草药, 2000(31): 458-461.
[6] Abdel-Hady, M.S., Okasha, E.M., Soliman, S.S.A. and Talaat, M. (2008) Effect of Gamma Radiation and Gibberellic Acid on Germination and Alkaloid Production in Atropa belladonna L. Australian Journal of Basic and Applied Sciences, 2, 401-405.
[7] 杨宝成. 苍术产销分析[J]. 特种经济动植物, 2013(11): 19-21.
[8] 宗世贤, 袁昌齐. 江苏省稀有濒危药用植物的现状和保护[J]. 中国野生植物资源, 1996(1): 1-5.
[9] 胡世林. 现代道地论概要[J]. 中国中医药信息杂志, 1995(7): 7-9.
[10] 张燕, 杨光, 陈美兰, 朱寿东, 王凌, 郭兰萍. 不同小生境对苍术生长和4种挥发油的影响[J]. 中国中药杂志, 2015(21): 4142-4148.
[11] 郭兰萍, 黄璐琦, 阎洪, 吕冬梅, 蒋有绪. 基于地理信息系统的苍术道地药材气候生态特征研究[J]. 中国中药杂志, 2005, 30(8): 565-569.
[12] 郭兰萍, 黄璐琦, 蒋有绪, 刘旭拢, 潘耀忠, 吕冬梅, 张晴. 影响苍术挥发油组分的气候主导因子及气候适宜性区划研究[J]. 中国中药杂志, 2007(32): 888-893.
[13] 贺善安, 冈田稔, 等. 茅苍术资源的保护和利用[J]. 植物资源与环境学报, 1993(1): 1-6.
[14] 国家药典委员会. 中华人民共和国药典[M]. 北京: 化学工业出版社, 2005: 128.
[15] Yuan, J., Zhou, J.-Y., Li, X. and Dai, C.-C. (2016) The Primary Mechanism of Endophytic Fungus Gilmaniella sp. AL12 Promotion of Plant Growth and Sesquiterpenoid Accumulation in Atractylodes lancea. Plant Cell, Tissue and Organ Culture (PCTOC), 1-14.
http://dx.doi.org/10.1007/s11240-016-0971-z
[16] 王宇, 戴传超, 陈晏. 茅苍术挥发油对三种内生真菌及七种外源真菌的抑菌活性[J]. 应用生态学报, 2009(11): 2778-2784.
[17] 张明发, 沈雅琴, 朱自平, 王红武. 苍术药理研究[J]. 时珍国医国药, 1999, 10(1): 1-3.
[18] 詹丹丹, 张颖. 浅谈苍术药理作用及质量控制的研究进展[J]. 黑龙江医药, 2012, 25(3): 459-459.
[19] 周军, 赵晨, 王杰. 藿香正气不同制剂中苍术素的含量测定[J]. 药物分析杂志, 2012(8): 037.
[20] 杨冬丽, 王春民, 刘井利, 徐晓伟, 李瑞敏, 王成. 颈复康制剂挥发油中苍术素含量测定及其在不同介质中稳定性研究[J]. 中国中医药信息杂志, 2013, 20(7): 55-57.
[21] 宋小珍. 藿香、苍术提取物复合制剂对高温应激猪小肠消化吸收的影响[D]: [博士学位论文]. 南京: 南京农业大学, 2008.
[22] Chu, S.S., Jiang, G.H. and Liu, Z.L. (2011) Insecticidal Compounds from the Essential Oil of Chinese Medicinal Herb Atractylodes chinensis. Pest Management Science, 67, 1253-1257.
http://dx.doi.org/10.1002/ps.2180
[23] Chen, Y., Wu, Y., Wang, H. and Gao, K. (2012) A New 9-Nor-Atractylodin from Atractylodes lancea and the Antibacterial Activity of the Atractylodin Derivatives. Fitoterapia, 83, 199-203.
http://dx.doi.org/10.1016/j.fitote.2011.10.015
[24] 彭华胜, 王德群. 南苍术与野生白术的开花动态研究[J]. 现代中药研究与实践, 2007, 21(3): 20-22.
[25] 朴锦, 王坤, 严一字, 具红光. 不同育苗措施对关苍术出苗及幼苗质量的影响[J]. 北方园艺, 2013(19): 164-167.
[26] 张波, 王宏伟, 肖逸, 鞠群, 戴传超. 浸种及接种内生真菌对茅苍术种子发芽与幼苗生长的影响[J]. 江苏农业科学, 2012, 40(9): 227-230.
[27] 徐友贵, 王小明, 田中联, 魏大为. 茅苍术根结线虫病的调查与防治研究[J]. 中药材, 1991(10): 10-12.
[28] 徐友贵, 苏筱娟. 茅苍术病害的防治研究[J]. 中药材, 1990(7): 9-11.
[29] Zhou, J., Zhao, X. and Dai, C. (2014) Antagonistic Mechanisms of Endophytic Pseudomonas fluorescens against Athelia rolfsii. Journal of Applied Microbiology, 117, 1144-1158.
http://dx.doi.org/10.1111/jam.12586
[30] Vickers, C.E., Bongers, M., Liu, Q., Delatte, T. and Bouwmeester, H. (2014) Metabolic Engineering of Volatile Isoprenoids in Plants and Microbes. Plant, Cell & Environment, 37, 1753-1775.
http://dx.doi.org/10.1111/pce.12316
[31] Rao, S.R. and Ravishankar, G.A. (2002) Plant Cell Cultures: Chemical Factories of Secondary Metabolites. Biotechnology Advances, 20, 101-153.
http://dx.doi.org/10.1016/S0734-9750(02)00007-1
[32] 李西腾, 吴沿友. 茅苍术的组织培养和快速繁殖[J]. 广西热带农业, 2006(2): 33-34.
[33] 巢建国, 谈献和, 张瑜, 张莹, 庄森. 茅苍术快速繁殖[J]. 中药材, 2001, 24(7).
[34] 刘海萍, 巢建国. 药用植物茅苍术的组织培养[J]. 现代中药研究与实践, 2005, 19(5): 11-13.
[35] 李文, 谢烨明, 罗翠平, 李金华, 曾万勇. 正交设计优化南苍术快速繁殖培养基的研究[J]. 安徽农业科学, 2012, 40(2): 663-666.
[36] 宋艳娇, 吴沿友, 朱咏莉, 施倩倩. 不同类型茅苍术组培过程中蔗糖含量的无菌动态检测[J]. 江苏农业科学, 2011(1): 68-69.
[37] 赵玉国, 吴沿友, 桑小花. 茅苍术胚培养与快速繁殖(简报)[J]. 亚热带植物科学, 2007, 36(3): 64.
[38] Yu, K.-W., Gao, W.Y., Hahn, E.-J. and Paek, K.-Y. (2001) Effects of Macro Elements and Nitrogen Source on Adventitious root Growth and Ginsenoside Production in Ginseng (Panax ginseng CA Meyer). Journal of Plant Biology, 44, 179-184.
http://dx.doi.org/10.1007/BF03030349
[39] Nemhauser, J.L., Hong, F. and Chory, J. (2006) Different Plant Hormones Regulate Similar Processes through Largely Nonoverlapping Transcriptional Responses. Cell, 126, 467-475.
http://dx.doi.org/10.1016/j.cell.2006.05.050
[40] 蒋细旺. 植物细胞工程[M]. 北京: 经济科学出版社, 2009.
[41] Jeandet, P., Clément, C. and Courot, E. (2014) Resveratrol Production at Large Scale Using Plant Cell Suspensions. Engineering in Life Sciences, 14, 622-632.
http://dx.doi.org/10.1002/elsc.201400022
[42] Cha, M., Shim, S.H., Kim, S.H., Kim, O.T., Lee, S.W., Kwon, S.Y. and Baek, K.H. (2012) Production of Taxadiene from Cultured Ginseng Roots Transformed with Taxadiene Synthase Gene. BMB Reports, 45, 589-594.
http://dx.doi.org/10.5483/BMBRep.2012.45.10.085
[43] 方芳, 戴传超, 张波, 梁侨丽. 茅苍术悬浮细胞系建立及内生真菌诱导子对其挥发油积累的影响[J]. 中草药, 2009, 40(3): 452-455.
[44] Fatima, S., Mujib, A. and Tonk, D. (2015) NaCl Amendment Improves Vinblastine and Vincristine Synthesis in Catharanthus roseus: A Case of Stress Signalling as Evidenced by Antioxidant Enzymes Activities. Plant Cell, Tissue and Organ Culture (PCTOC), 121, 445-458.
http://dx.doi.org/10.1007/s11240-015-0715-5
[45] Durante, M., Caretto, S., Quarta, A., De Paolis, A., Nisi, R. and Mita, G. (2011) β-Cyclodextrins Enhance Artemisinin Production in Artemisia annua Suspension Cell Cultures. Applied Microbiology and Biotechnology, 90, 1905-1913.
http://dx.doi.org/10.1007/s00253-011-3232-4
[46] Zhang, R., Zhang, B.-L., Li, G.-C., Xie, T., Hu, T. and Luo, Z.-Y. (2015) Enhancement of Ginsenoside Rg1 in Panax ginseng Hairy Root by Overexpressing the α-L-Rhamnosidase Gene from Bifidobacterium breve. Biotechnology Letters, 37, 2091-2096.
http://dx.doi.org/10.1007/s10529-015-1889-y
[47] 袁媛, 吕冬梅, 黄璐琦, 郭兰萍. 苍术不定根诱导培养的研究[J]. 中国中药杂志, 2007, 32(1): 65-66.
[48] Murthy, H.N., Lee, E.-J. and Paek, K.-Y. (2014) Production of Sec-ondary Metabolites from Cell and Organ Cultures: Strategies and Approaches for Biomass Improvement and Metabolite Accumulation. Plant Cell, Tissue and Organ Culture (PCTOC), 118, 1-16.
http://dx.doi.org/10.1007/s11240-014-0467-7
[49] 郭兰萍, 黄璐琦, 阎玉凝. 土壤中无机元素对茅苍术道地性的影响[J]. 中国中药杂志, 2002, 27(4): 245-250.
[50] Expósito, O., Bonfill, M., Onrubia, M., Jané, A., Moyano, E., Cusidó, R.M., Palazón, J. and Piñol, M.T. (2009) Effect of Taxol Feeding on Taxol and Related Taxane Production in Taxus baccata Suspension Cultures. New Biotechnology, 25, 252-259.
http://dx.doi.org/10.1016/j.nbt.2008.11.001
[51] Verma, P., Khan, S.A., Mathur, A.K., Ghosh, S., Shanker, K. and Kalra, A. (2014) Improved Sanguinarine Production via Biotic and Abiotic Elicitations and Precursor Feeding in Cell Suspensions of Latex-Less Variety of Papaver somniferum with Their Gene Expression Studies and Upscaling in Bioreactor. Protoplasma, 251, 1359-1371.
http://dx.doi.org/10.1007/s00709-014-0638-8
[52] Almagro, L., Perez, A.J.L. and Pedreño, M.A. (2011) New Method to Enhance Ajmalicine Production in Catharanthus roseus Cell Cultures Based on the Use of Cyclodextrins. Biotechnology Letters, 33, 381-385.
http://dx.doi.org/10.1007/s10529-010-0430-6
[53] Yuan, Y., Liu, Y.J., Huang, L.Q., Cui, G.H. and Fu, G.F. (2008) Soil Acidity Elevates Some Phytohormone and β- Eudesmol Contents in Roots of Atractylodes lancea. Russian Journal of Plant Physiology, 56, 133-137.
http://dx.doi.org/10.1134/S1021443709010191
[54] Pazzagli, L., Seidl-Seiboth, V., Barsottini, M., Vargas, W.A., Scala, A. and Mukherjee, P.K. (2014) Cerato-Platanins: Elicitors and Effectors. Plant Science, 228, 79-87.
http://dx.doi.org/10.1016/j.plantsci.2014.02.009
[55] Bahabadi, S.E., Sharifi, M., Chashmi, N.A., Murata, J. and Satake, H. (2014) Significant Enhancement of Lignan Accumulation in Hairy Root Cultures of Linum album Using Biotic Elicitors. Acta Physiologiae Plantarum, 36, 3325- 3331.
http://dx.doi.org/10.1007/s11738-014-1700-z
[56] 吕立新, 王宏伟, 梁雪飞, 郝思静, 杜威, 朱虹, 戴传超. 不同化学型和季节变化对茅苍术内生真菌群落多样性的影响[J]. 生态学报, 2014, 34(24): 7300-7310.
[57] 陈佳昕, 戴传超, 李霞, 田林双, 谢慧. 茅苍术内生真菌的分离鉴定及在组培苗中的回接[J]. 广西植物, 2008, 28(2): 256-260.
[58] 张波, 戴传超, 方芳, 陈佳昕. 三种内生真菌对茅苍术组培苗的生长及主要挥发油成分的影响[J]. 生态学杂志, 2009, 28(4): 704-709.
[59] 张霁, 刘大会, 郭兰萍, 金航, 杨光, 周洁. 不同温度下丛枝菌根对苍术根茎生物量和挥发油的影响[J]. 中草药, 2011, 42(2): 372-375.
[60] 陶金华, 汪冬庚, 濮雪莲, 赵喜, 江曙. H2O2介导内生真菌诱导子促进茅苍术细胞HMGR的活化和β-枝叶醇的生物合成[J]. 中草药, 2013, 44(19): 2740-2744.
[61] Ren, C.G., Chen, Y. and Dai, C.C. (2014) Erra-tum to: Cross-Talk between Calcium-Calmodulin and Brassinolide for Fungal Endophyte-Induced Volatile Oil Accu-mulation of Atractylodes lancea Plantlets. Journal of Plant Growth Regulation, 33, 903-903.
http://dx.doi.org/10.1007/s00344-014-9430-4
[62] Wang, Y., Dai, C.C., Zhao, Y.W. and Peng, Y. (2011) Fungal Endophyte-Induced Volatile Oil Accumulation in Atractylodes lancea Plantlets Is Mediated by Nitric Oxide, Salicylic Acid and Hydrogen Peroxide. Process Biochemistry, 46, 730-735.
http://dx.doi.org/10.1016/j.procbio.2010.11.020
[63] 陶金华, 濮雪莲, 江曙. 内生真菌诱导子对茅苍术细胞生长及苍术素积累的影响[J]. 中国中药杂志, 2011, 36(1): 27-31.
[64] Ren, C.G. and Dai, C.C. (2013) Nitric Oxide and Brassinosteroids Mediated Fungal Endophyte-Induced Volatile Oil Production through Protein Phosphorylation Pathways in Atractylodes lancea Plantlets. Journal of Integrative Plant Biology, 55, 1136-1146.
http://dx.doi.org/10.1111/jipb.12087
[65] Wang, X.M., Yang, B., Ren, C.G., Wang, H.W., Wang, J.Y. and Dai, C.C. (2015) Involvement of Abscisic Acid and Salicylic Acid in Signal Cascade Regulating Bacterial Endophyte-Induced Volatile Oil Biosynthesis in Plantlets of Atractylodes lancea. Physiologia Plantarum, 153, 30-42.
http://dx.doi.org/10.1111/ppl.12236
[66] Mahmoud, S.S. and Croteau, R.B. (2001) Metabolic Engineering of Es-sential Oil Yield and Composition in Mint by Altering Expression of Deoxyxylulose Phosphate Reductoisomerase and Menthofuran Synthase. Proceedings of the National Academy of Sciences of the United States of America, 98, 8915-8920.
http://dx.doi.org/10.1073/pnas.141237298
[67] Botella-Pavía, P. and Rodríguez-Concepción, M. (2006) Carotenoid Biotechnology in Plants for Nutritionally Improved Foods. Physiologia Plantarum, 126, 369-381.
http://dx.doi.org/10.1111/j.1399-3054.2006.00632.x
[68] 王凌健, 方欣, 杨长青, 李建戌, 陈晓亚. 植物萜类次生代谢及其调控[J]. 中国科学: 生命科学, 2013(12): 1030- 1046.
[69] Lu, X., Zhang, L., Zhang, F., Jiang, W., Shen, Q., Zhang, L., Lv, Z., Wang, G. and Tang, K. (2013) AaORA, a Trichome-Specific AP2/ERF Transcription Factor of Artemisia annua, Is a Positive Regulator in the Artemisinin Biosynthetic Pathway and in Disease Resistance to Botrytis cinerea. New Phytologist, 198, 1191-1202.
http://dx.doi.org/10.1111/nph.12207
[70] Broun, P. and Somerville, C. (2001) Progress in Plant Metabolic Engi-neering. Proceedings of the National Academy of Sciences of the United States of America, 98, 8925-8927.
http://dx.doi.org/10.1073/pnas.171310598
[71] Liu, Q., Majdi, M., Cankar, K., Goedbloed, M., Charnikhova, T., Verstappen, F.W., de Vos, R.C., Beekwilder, J., van der Krol, S. and Bouwmeester, H.J. (2011) Reconstitution of the Costunolide Biosynthetic Pathway in Yeast and Nicotiana benthamiana. PLoS ONE, 6, e23255.
http://dx.doi.org/10.1371/journal.pone.0023255
[72] 黎万奎, 胡之璧. 内生菌与天然药物[J]. 中国天然药物, 2005, 3(4): 193-199.
[73] Ignea, C., Cvetkovic, I., Loupassaki, S., Kefalas, P., Johnson, C.B., Kampranis, S.C. and Makris, A.M. (2011) Improving Yeast Strains Using Recyclable Integration Cassettes, for the Production of Plant Ter-penoids. Microbial Cell Factories, 10, 4.
http://dx.doi.org/10.1186/1475-2859-10-4
[74] Paddon, C.J. and Keasling, J.D. (2014) Semi-Synthetic Artemisinin: A Model for the Use of Synthetic Biology in Pharmaceutical Development. Nature Reviews Microbiology, 12, 355-367.
http://dx.doi.org/10.1038/nrmicro3240