食管鳞癌组织中nm23-H1和E-cadherin的表达及临床意义

The Expression and Clinical Significance of nm23-H1 and E-Cadherin in Esophageal Squamous Cell Carcinomas

摘要: 目的：研究转移抑制基因(nm23-H1)和E-钙粘附蛋白(E-cadherin)在食管鳞癌组织中表达情况，了解两者之间的相关性及其与临床病理特征的关系。方法：采用免疫组化EnVision法检测60例食管鳞癌组织中nm23-H1和E-cadherin的表达情况，比较其相关性。结果：食管鳞癌组织中nm23-H1和E-cadherin的阳性表达率分别为38.3% (23/60)和41.7% (25/60)，正常食管黏膜组织中nm23-H1和E-cadherin的阳性表达均为90.0% (9/10)，nm23-H1和E-cadherin在食管鳞癌组织中的阳性表达率显著低于正常食管黏膜组织。nm23-H1、E-cadherin表达与食管癌患者的年龄、性别、组织类型无关(P > 0.05)，而与肿瘤分化程度、浸润深度、区域淋巴结转移及TNM分期有关(P < 0.05)。nm23-H1和E-cadherin在食管鳞癌组织中的表达呈显著正相关。结论：nm23-H1和E-cadherin在食管鳞癌中的表达均为低表达，并明显低于正常食管粘膜。nm23-H1和E-cadherin的异常表达与肿瘤分化程度、浸润深度、淋巴结转移及临床分期关系密切，nm23-H1和E-cadherin可以作为判定食管鳞癌恶性程度及转移等生物学行为的参考指标。

Abstract: Objective: The objective is to investigate the expressions of nm23-H1 and E-cadherin in esophageal squamous cell carcinomas, and to explore the correlation between the two and its relationship with clinical pathological features. Methods: The expressions of nm23-H1 and E-cadherin in the tumor tissues of 60 patients with esophageal squamous cell carcinomas were examined immuno-histochemistrically by the EnVision method, and their relationships were compared. Results: The positive rates of nm23-H1 and E-cadherin in expression in the esophageal squamous cell car-cinomas tissue were 38.3% (23/60) and 41.7% (25/60), respectively. The positive rates of nm23-H1 and E-cadherin in expression in the normal esophageal mucosa were 90.0% (9/10). The positive expression rates of nm23-H1 and E-cadherin in esophageal squamous cell carcinomas were significantly lower than those in normal esophageal mucosa. The expression of nm23-H1 and E-cadherin had no significant correlation with the patients’ age, sex, histological type (P > O.05), but had correlation with degree of the differentiation, depth of tumor invasion, regional lymph node metastasis and TNM stage (P < 0.05). Nm23-H1 had positive correlation with E-cadherin. Conclusions: The expression of nm23-H1 and E-cadherin in esophageal squamous cell carcinomas was obviously lower than that in normal esophageal mucosa. The abnormal expression of nm23-H1 and E-cadherin are closely related to the degree of the differentiation of the cancer, depth of tumor invasion, lymph node metastasis and clinical stage; nm23-H1 and E-cadherin may be used as reference for assessing the malignancy and metastasis of esophageal squamous cell carcinomas.