雌激素、胰岛素诱导子宫内膜癌细胞中VEGF和bFGF的表达及临床意义

The Expression and Clinical Significance of VEGF and bFGF in Endometrial Carcinoma

摘要: 初步探讨雌二醇、胰岛素诱导子宫内膜癌细胞系Ishikawa细胞中血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的产生及临床意义。采用荧光定量PCR技术分别检测雌二醇、胰岛素、雌二醇 + 胰岛素作用后Ishikawa细胞中VEGF、bFGF基因表达的情况。结果显示相同浓度雌二醇(1 × 10⁻⁸ mol/L)，胰岛素(1 × 10⁻⁸ mol/L)，雌二醇 + 胰岛素(1 × 10⁻⁸ mol/L雌二醇 + 1 × 10⁻⁸ mol/L胰岛素)对Ishikawa细胞中VEGF、bFGF均有不同程度促进表达作用；VEGF、bFGF基因在雌二醇+胰岛素组中表达与雌二醇、胰岛素组比较无明显增高，差异没有统计学意义(p > 0.05)。因此，雌激素、胰岛素诱导子宫内膜癌细胞系Ishikawa细胞产生VEGF、bFGF，促进了子宫内膜癌发生、发展。

Abstract: The aim of this article is to explore the expression and clinical significance of VEGF and bFGF in endometrial carcinoma. PCR technique was used to detect the variation of expression of VEGF and bFGF in estradiol, insulin and both adding group between Ishikawa cells. Estradiol, insulin, estradiol insulin have different degrees of promoting the expression of VEGF and bFGF at the same concentration of E2, Insulin, E2 + Insulin on Ishikawa cells; the expression of VEGF and bFGF in E2 + Insulin cell group in the same time point shows no significant increase than E2, Insulin cell group. The difference was not statistically significant (p > 0.05). VEGF and bFGF are important angiogenesis factors in endometrial carcinoma. Estrogen and insulin induced Ishikawa cells produce VEGF and bFGF and promote the occurrence and development of endometrial carcinoma.