巢式PCR检测白斑综合征病毒在白纹伊蚊幼虫体内的滞留时间

Detention Time of White Spot Syndrome Virus in the Body of Aedes albopictus Larva by Using Nest PCR Detection

摘要: 白斑综合征病毒(WSSV)是对全球虾类养殖业危害最大，传播最广的病毒之一，至今已经造成了数十亿美元的损失。克氏原螯虾的养殖也受到WSSV的威胁，陆续有养殖的克氏原螯虾因感染WSSV而大量死亡的报道。通过野外采样在杭州周边的养殖区均有发现克氏原螯虾携带有WSSV，因此对WSSV在淡水生态系统中的传播途径值得深入研究。白纹伊蚊既是一种攻击性很强的蚊子，也是一种重要的病毒媒介，它可以传播很多病原体，包括登革热、罗斯河病毒和西尼罗病毒。白纹伊蚊作为淡水水体生态系统中的重要一环，其幼虫携带白斑综合征病毒的研究仍未见报道。本试验通过巢式PCR技术检测出白斑综合征病毒在白纹伊蚊幼虫体内的滞留时间在48 h左右。本研究为WSSV在野外淡水水体中的传播媒介研究提供了新的证据，为防治克氏原螯虾感染白斑综合征提供了新的科学依据。

Abstract: White spot syndrome virus (WSSV), which causes high mortality in many economic shrimp aquacul-ture, and is widespread over the world, makes the large economic losses to the shrimp industry in China. In recent years, Procambarus clarkii farming is also threatened by WSSV. Gradually, there are many reports about the massive dead of P. clarkii because of the infection of WSSV. P. clarkii in aqui-culture area around Hangzhou was found to carry WSSV through field sampling. Therefore, the route of transmission of WSSV in freshwater ecosystems is worth studying deeply. Aedes albopictus not only has very strong aggressivity, but also is an epidemiologically important vector for the transmission of many viral pathogens, including the dengue fever, Ross River virus and West Nile virus. Although A. albopictus is an important part of the fresh water ecological system, the research of A. albopictus larvae with white spot syndrome virus has not been reported. This test detects that the detention time of white spot syndrome virus in the body of A. albopictus larva is 48 h by using Nest PCR detection. This study provides new evidence for the transmission of WSSV in fresh water and provides a new scientific basis for the prevention Procambarus clarkii from white spot syndrome.