温栀子对溃疡性结肠炎治疗作用的研究

Research on the Therapeutic Effects of Wen Gardenia on Ulcerative Colitis

摘要: 目的：探讨温栀子对溃疡性结肠炎的治疗效果及合适的治疗剂量。方法：48只SD大鼠采用DNCB/乙酸复合法建立溃疡性结肠炎模型，确定造模成功后，随机分成低、中、高剂量温栀子组、SASP阳性对照组及生理盐水对照组，分别予以相应治疗。从形态学、免疫学和病理学角度探究温栀子对溃疡性结肠炎的治疗作用。结果：经治疗，低、中、高剂量温栀子组大鼠症状、体征较生理盐水组均有好转，其中高剂量温栀子组溃疡性结肠炎改善最为明显，扫描电镜下显示结肠细胞表面平滑；病理切片显示温栀子治疗组黏膜结构均有所恢复；透射电镜下可见细胞结构恢复良好。其大体形态学评分、病理组织学等指标均较模型组有显著差异(P < 0.05)。结论：温栀子是治疗溃疡性结肠炎的有效药物。

Abstract: Objective: To explore the therapeutic effect of Wen Gardenia Ellis dosage on ulcerative colitis and appropriate treatment dose. Method: 48 SD rats ulcerative colitis model were inoculated using DNCB/acetic acid method. After determining the successful models, they were randomly divided into low, medium and high dose dosage Wen Gardenia group, SASP positive control group and saline control group which were given their corresponding treatments. The morphology, immunology and pathology findings were used to explore the dosage of Wen Gardenia Ellis on ulcerative colitis. Re-sults: After the treatment, the group of rats treated with Wen gardenia had symptoms and signs which were improved when compared to the saline group, and the high dose group had the most significant improvement in the treatment of ulcerative colitis; under scanning electron microscope: colonic cell surface was smooth; pathology showed that the mucosal structure of rats that had the Wen Gardenia treatment was restored; cell structure was observed under transmission electron microscope and exhibited good recovery. The general morphology, histopathological score and other indicators were compared with the model group and the results showed that they were significantly different (P < 0.05). Conclusion: The dose of Wen Gardenia is an effective drug in the treatment of ulcerative colitis.