人血清中游离氨基酸指纹图谱的初步研究

A Preliminary Study on Free Amino Acid Fingerprint of Human Serum

摘要: 目的：建立人血清中游离氨基酸的指纹图谱。方法：采用L-8900全自动氨基酸分析仪测定健康人群血清中的游离氨基酸，选择15个主要特征峰为指纹图谱共有峰。结果：建立了12批人血清样品的游离氨基酸指纹图谱。结论：建立的指纹图谱有较好的精密度、重复性和稳定性，可作为人血清游离氨基酸指纹图谱库的理论基础。

Abstract: Object: To establish free amino acid fingerprint of human serum. Method: The free amino acid of serum from the healthy persons was determined by the L-8900 automatic amino acid analyzer, and 15 major characteristic peaks of fingerprint were chosen as the common peak. Result: Free amino acid fingerprint of 12 batches of human serum was established. Conclusion: The fingerprint has preferably precision, repeatability and stability; it can be used as the theoretical basis of free amino acid fingerprint database of human serum.