Ti-O膜表面固定不同手性酒石酸及其血液相容性评价

Immobilization of Different Chiral Tartaric Acid on Ti-O Surface and Blood Compatibility Evaluation

摘要: 手性是生命体系的基本特征，生命体系具有高度的手性选择性，表面手性可能显著影响生物材料的生物学功能。本文将不同手性酒石酸分子固定在Ti-O膜表面，获得了不同手性表面。XPS检测结果显示，沉积了多巴胺的样品表面出现了新的N1s峰，接枝了己二胺的样品表面N:C值升高，随后又因为接枝了手性酒石酸，其表面的O:C值升高，XPS结果表明各层次表面均已制备成功。水接触角检测结果显示，L-酒石酸表面和D-酒石酸表面的水接触角几乎没有差异，说明表面手性不会影响材料表面的亲疏水性。通过纤维蛋白原变性实验和体外血小板粘附与激活实验来评价手性对于材料表面血液相容性的影响。相对于L-酒石酸表面，D-酒石酸表面显示出较低的纤维蛋白原变性程度，并且血小板的粘附量更少，血小板被激活的程度更低。这些结果表明手性对于生物材料的抗凝血性能有着不容忽视的影响，为开发性能优异的抗凝血材料提供了新的思路。

Abstract: Chirality is one of the basic characteristics of life system, which has a high degree of chiral selectivity, surface chirality may significantly affect the biological function of biomaterials. In this paper, different chiral tartaric acid molecules were immobilized on the surface of Ti-O films. XPS test results showed the peak of nitrogen appeared on the samples which had deposited dopamine, the ratio of nitrogen to carbon was increased when hexamethylendiamine was introduced. When chiral tartaric acid was grafted, the ratio of oxygen to carbon was increased. XPS results showed that all surfaces had been successfully prepared. The water contact angle test results showed that no difference was present on the L-surface and D-surface, indicating that surface chirality had little effect on surface hydrophobicity. The effects of chirality on the blood compatibility of materials were evaluated by fibrinogen denaturation test and platelet adhesion and activation assay in vitro. Compared with L-tartaric acid surface, D-tartaric acid surface showed a lower degree of denatura-tion of fibrinogen, less platelet adhesion and a lower degree of platelet activation. These results indicate that chirality cannot be ignored in the anticoagulant properties of biomaterials, which provides a new idea for the development of anticoagulant materials with excellent properties.