喘鸣患儿外周血单个核CD14 CD105双阳性细胞测定初步研究

Preliminary Study of CD14 CD105 Double Positive Cells in Peripheral Blood of Patients with Wheezing in Children

摘要: 目的：采用外周血单个核上CD14 CD105双阳性细胞作为气道平滑肌干细胞表面标志，了解其与儿童喘鸣的关系，为哮喘的早期诊断提供依据。方法：随机选取5岁以下喘息的儿童，以同年龄组为对照组，用流式细胞仪测定外周血单个核CD14 CD105双阳性细胞。结果：35例喘鸣组患儿，CD14 CD105双阳性细胞为12.67% ± 5.56%，而30例对照组为4.84 ± 1.39%，有显著性意义(P < 0.01)。结论：喘息儿童中外周血CD14 CD105双阳性细胞比例显著高于正常对照组，提示儿童喘息与气道平滑肌干细胞增高之间可能有某种关联。

Abstract: Objective: Using CD14 CD105 double positive cells as the surface markers of airway smooth muscle stem cells in peripheral blood mononuclear cells is to understand the relationship between asthma and wheezing in children, and to provide evidence for the early diagnosis of asthma. Methods: Randomly selecting 5-year-old children with wheezing, in the same age group as the control group, the CD14 CD105 double positive cells were measured by flow cytometry. Results: The CD14 CD105 positive cells of 35 cases were 12.67% ± 5.56%, while the control group was 4.84 ± 1.39%, which was significant (P < 0.01). Conclusion: The proportion of CD14 CD105 positive cells in the peripheral blood of asthmatic children was significantly higher than that in the normal control group, which suggested that there might be some correlation between the children wheezing and the increase of airway smooth muscle stem cells.