基于倏逝波核酸适体生物传感器的赭曲霉毒素A的新型检测方法

A Novel Detection Method of Ochratoxin A with Evanescent Wave All-Fiber Aptamer Biosensor

摘要:

本文结合核酸适体的优异性能，利用前期研发的倏逝波光纤生物传感器(Evanescent Wave All-fiber Biosensor, EWAB)，发展了赭曲霉毒素A (OTA)的新型快速检测方法。检测方法基于核酸适体与污染物质的特异性结合能力，利用溶液中的OTA与光纤探头表面的核酸适体互补链共同竞争OTA核酸适体，建立了核酸适体结构竞争法检测OTA的标准曲线，线性检测区间为0.13 μg/L~2.89 μg/L，检测限为0.063 μg/L。并对实际样品进行了检测，标回收率在80%~120%之间。实验结果表明此方法检测OTA具有良好的灵敏度，检测方法简便快速，并可以较好的应用于实际样品检测中。 

In this study, using the aptamer with excellent properties, the new and rapid detection methods of Ochratoxin A (OTA) has been developed with an evanescent wave all-fiber biosensor (EWAB). The method based on the high affinity and specificity between pollutants and aptamer, and both complementary strands and OTA completely bound with Cy5.5 labeled OTA aptamer in solution. The quantitative detection of OTA was achieved. The linear detection range of OTA was 0.13 μg/L - 2.89 μg/L, and the detection limit was 0.063 μg/L. The recoveries of spiked samples were from 80% to 120%.This paper show a high sensitivity, simple and fast detection method.