唾液酸化Lewis X和半乳糖凝集素-3表达与甲状腺乳头状癌浸润转移的关系
Expression of Sialyl Lewis X and Galectin-3 in Papillary Thyroid Carcinoma and Their Relationships with Invasion and Metastasis
DOI: 10.12677/ACRPO.2017.64006, PDF, HTML, XML,  被引量 下载: 1,796  浏览: 3,820 
作者: 尚培中, 南润玲, 王 金, 聂阿娜, 李艳艳, 张 伟, 胡 玮, 谷化平：中国人民解放军第251医院普通外科，河北 张家口
关键词: 甲状腺肿瘤；唾液酸化Lewis X；半乳糖凝集素-3；转移；Thyroid Neoplasms； Sialyl Lewis X； Galectin-3； Metastasis
摘要: 目的：探讨唾液酸化Lewis X (SLeX)和半乳糖凝集素-3 (Galectin-3)在甲状腺乳头状癌中的表达及其与癌组织浸润转移的关系。方法：应用免疫组化染色EliVisionTM plus两步法检测164例甲状腺乳头状癌组织和30例癌旁甲状腺组织中SLeX和Galectin-3的表达状况。结果：癌组织SLeX和Galectin-3总体阳性表达率分别为70.7%和92.1%，癌旁甲状腺组织SLeX和Galectin-3总体阳性表达率分别为6.7%和20.0%，两者在癌组织总体阳性表达率显著高于癌旁甲状腺组织(P < 0.05)。有浸润患者SLeX和Galectin-3总体阳性表达率分别为84.8%和98.0%，无浸润患者分别为49.2%和83.1%，两者在有浸润患者中的总体阳性表达率均显著高于无浸润患者(P < 0.05)。有淋巴结转移患者SLeX和Galectin-3总体阳性表达率分别为89.2%和96.8%，无淋巴结转移患者分别为46.5%和85.9%，两者在有淋巴结转移患者中的总体阳性表达率均显著高于无淋巴结转移患者(P < 0.05)。癌组织SLeX与Galectin-3表达具有一定正相关性(r = 0.41)。结论：SLeX和Galectin-3在甲状腺乳头状癌组织中多数呈强阳性表达，两者与癌组织浸润转移密切相关。
Abstract: Objective: To investigate the expression of sialyl Lewis X (SLeX) and Galectin-3 in papillary thyroid carcinoma and their relationships with the potential of invasion and metastasis. Methods: The expression of SLeX and Galectin-3 in 164 cases of papillary thyroid carcinoma and 30 cases of para-tumorous tissue were studied by EliVisionTM plus two-step immunohistochemical method. Results: The positive rates of SLeX and Galectin-3 in papillary thyroid carcinoma were 70.7% and 92.1%; those in para-tumorous tissue were 6.7% and 20.0%. Both in papillary thyroid carcinoma were significantly higher than those in para-tumorous tissue (P < 0.05). The positive rates of SLeX and Galectin-3 in the patients with tumor invasion were 84.8% and 98.0%; those in the patients without tumor invasion were 49.2% and 83.1%. Both in the patients with tumor invasion were significantly higher than those in the patients without tumor invasion (P < 0.05). The positive rates of SLeX and Galectin-3 in the patients with lymphatic metastasis were 89.2% and 96.8%; those in the patients without lymphatic metastasis were 46.5% and 85.9%. Both in the patients with lymphatic metastasis were significantly higher than those in the patients without lymphatic metastasis (P < 0.05). There was a positive correlation between SLeX and Galectin-3 expression in papillary thyroid carcinoma (r = 0.41). Conclusion: High level expression of SLeX and Galectin-3 in papillary thyroid carcinoma were closely correlated with the invasive and metastasic potential.
文章引用：尚培中, 南润玲, 王金, 聂阿娜, 李艳艳, 张伟, 胡玮, 谷化平. 唾液酸化Lewis X和半乳糖凝集素-3表达与甲状腺乳头状癌浸润转移的关系[J]. 亚洲肿瘤科病例研究, 2017, 6(4): 33-39. https://doi.org/10.12677/ACRPO.2017.64006

参考文献

[1] 马艳飞, 秦华东, 石铁锋, 等. 甲状腺乳头状癌中央区食道旁淋巴结清扫的临床意义[J]. 中国现代普通外科进展, 2014, 17(6): 465-466.
[2] 尚培中, 柳勇, 南润玲. 甲状腺乳头状癌的诊断与治疗[J]. 中国现代普通外科进展, 2015, 18 (10): 833-836.
[3] 杨得琳, 邓小雨. 微小乳头状癌淋巴结转移及预防性淋巴结清扫的价值[J/CD]. 中华普外科手术学杂志(电子版), 2017, 11(3): 261-264.
[4] 尚培中, 张金江, 贾国洪, 等. 基质金属蛋白酶-2和唾液酸化 Lewis-X在乳腺癌中的表达及其意义[J/CD]. 中华乳腺病杂志(电子版), 2008, 2(2): 177-183.
[5] 尚培中, 王兵, 万立, 等. SLeX和p16在甲状腺乳头状癌中的表达及其意义[J]. 中华内分泌外科杂志, 2009, 3(3): 151-153.
[6] Sumana, B.S., Shashidhar, S. and Shivarudrappa, A.S. (2015) Galectin-3 Immunohistochemical Expression in Thyroid Neoplasms. Journal of Clinical and Diagnostic Research, 9, 7-11.
https://doi.org/10.7860/JCDR/2015/16277.6760
[7] 徐跃, 杨仙玉. 半乳糖凝集素-3及其相关抑制剂的研究进展[J]. 中国生化药物杂志, 2011, 32 (5): 417-421.
[8] 臧盛兵, 黄爱民, 刘景丰, 等. 肝细胞癌组织芯片环氧合酶-2表达的半定量图像分析[J]. 福建医科大学学报, 2008, 42(1): 23-25, 59.
[9] 高建芝, 杜经丽, 李佳, 等. VEGF相关信号通路在肝癌组织中的表达及临床意义[J]. 临床与实验病理学杂志, 2014, 30(1): 75-78.
[10] 王玉兰, 杜经丽, 石怀银, 等. Cyclin D1、p21WAF1、p53及Ki-67在肝细胞癌中的表达及与预后的关系[J]. 解放军医学杂志, 2014, 39(1): 20-24.
[11] 尚培中, 柳勇, 李永庆, 等. COX-2和Gal-3表达与甲状腺乳头状癌浸润转移的关系[J]. 河北北方学院学报(自然科学版), 2016, 32(8): 1-4.
[12] 尚培中, 王志湖, 李永庆, 等. 肝细胞癌CD44v6和FN表达及其意义[J]. 亚洲肿瘤科病例研究, 2017, 6(3): 15-20.
[13] 尚培中, 苗建军, 徐志勇, 等. 甲状腺乳头状癌157例手术治疗体会[J]. 中国现代普通外科进展, 2012, 15(8): 649-651.
[14] 尚培中, 张进权, 枊勇. 分化型甲状腺癌的诊断与规范化手术治疗[J]. 临床误诊误治, 2015, 28(6): 44-47.
[15] 史衍辉, 夏璐, 南林, 等. 甲状腺术式对术后低钙血症的影响[J]. 亚洲外科手术病例研究, 2016, 5(3): 17-20.
[16] 项田岗, 丁建. 纳米碳示踪技术在甲状腺癌根治术中的应用[J]. 中国现代普通外科进展, 2017, 20(4): 322-324.
[17] 万立, 尚培中, 张金江, 等. 基质金属蛋白酶-7在甲状腺良恶性病变组织中的表达及其意义[J]. 中国医师杂志, 2010, 12(2): 175-177.
[18] 于芳, 赵振宇, 贺青卿, 等. 甲状腺癌再次手术范围探讨及对策[J]. 中国现代普通外科进展, 2014, 17(7): 570-571, 582.
[19] 陈传萍, 何群, 王邵成, 等. TNF-α对人肝癌细胞系CD15和CD15s含量及细胞侵袭性的影响[J]. 中国医科大学学报, 2012, 41(11): 972-976.
[20] 董桂银. 唾液酸化Lewis X表达对三阴性乳腺癌复发的影响[J]. 重庆医学, 2014, 43(28): 3741-3743.
[21] Ohta, M., Ookoshi, T., Naiki, H., et al. (2015) HBME-1 and CD15 Immunocytochemistry in the Follicular Variant of Thyroid Papillary Carcinoma. Pathology International, 65, 119-125.
https://doi.org/10.1111/pin.12252
[22] Miyara, M., Chader, D., Sage, E., et al. (2015) Sialyl Lewis x (CD15s) Identifies Highly Differentiated and Most Suppressive FOXP3high Regulatory T Cells in Humans. PNAS, 112, 7225-7230.
https://doi.org/10.1073/pnas.1508224112
[23] 王首星, 尚培中. 结直肠癌Lewis-Selectin转移途径与西咪替丁抗癌治疗研究进展[J]. 中国普外基础与临床杂志, 2010, 17(7): 760-764.
[24] 王干, 刘佳维, 梁启超, 等. GALECTIN-3与细胞凋亡[J]. 牡丹江医学院学报, 2015, 36 (2): 101-103.
[25] 南润玲, 尚培中, 谷化平, 等. 半乳糖凝集素3、细胞角蛋白19及甲状腺过氧化物酶在甲状腺乳头状癌中的表达及意义[J/CD]. 中华临床医师杂志(电子版), 2013, 7(6): 2381-2384.
[26] 王士娜, 王星, 王翠芳. 甲状腺微小乳头状癌的临床病理特征及诊断标志物的应用[J]. 中国医科大学学报, 2014, 43(11): 1051-1053.
[27] 徐元成, 余丽, 郑娟. HBME-1、CD56、CK19、Gal-3及34ßE12在PTC中的表达及诊断价值[J]. 实用肿瘤杂志, 2016, 31(3): 267-270.
[28] Zheng, L., Zhao, M., Hu, X.Y., et al. (2016) Clinical Significance of HBME-1, Galectin-3, and CK19 Expression and the Status of BRAF Mutation in Papillary Thyroid Carcinoma. OTM, 2, 174-178.
[29] 武文娟, 崔灿, 康品方, 等. SLeX对肝癌HepG2细胞迁移和侵袭的影响[J]. 中国病理生理杂志, 2017, 33(4): 688-693.
[30] 温子海, 吕巧云. 丙胺酰谷氨酰胺注射液对食管癌术后患者半乳糖凝集素-3、D-二聚体及环氧合酶-2的影响分析[J]. 实用癌症杂志, 2017, 32(3): 398-400, 404.