外源激素对桂北地区原生国兰组培快繁的效应探究

Explore the Effect of Exogenous Hormones on Rapid Propagation of Tissue Culture of Native Orchids in Northern Guangxi

摘要: 利用L₉(3⁴)正交试验方法，研究IBA、6-BA两种外源激素和活性炭(AC)对桂北地区原生国兰组培快繁的效应。原球茎诱导形成阶段适宜培养基为MS + 6-BA 1.0 mg/L + IBA 0.6 mg/L + AC 4.0 mg/L；原球茎增殖期适宜培养基为MS + 6-BA 1.0 mg/L + IBA 0.4 mg/L + AC 4.0 g/L；原球茎分化期适宜培养基为MS + 6-BA 0.4 mg/L + IBA 0.6 mg/L + AC 0.5 g/L；壮苗及生根阶段适宜培养基为1/2MS + IBA 0.4 mg/L + AC 1.0 g/L。

Abstract: The effect of IBA and 6-BA two kinds of exogenous hormones and active carbon (AC) on rapid propagation of northern Guangxi native orchid tissue culture was studied by L₉ (3⁴) orthogonal test. At the induction stage of protocorms formation, the suitable medium was MS + 6-BA 1.0 mg/L + IBA 0.6 mg/L + AC 4.0 mg/L; at the proliferative stage of protocorms, the suitable medium was MS + 6-BA 1.0 mg/L + IBA 0.4 mg/L + AC 4.0 g/L; in the differentiation stage of protocorms, the suitable medium was MS + 6-BA 0.4 mg/L + IBA 0.6 mg/L + AC 0.5 g/L; in the seedling and rooting stage, the suitable medium was 1/2MS + IBA 0.4 mg/L + AC 1.0 g/L.