组织培养对沙柳器官形成与植株再生的影响研究

Effect of Tissue Culture Conditions on Organogenesis and Replant of Salix psammophila

摘要: 沙柳(Salix psammophila)是北方干旱和沙漠地区重要的生态经济植物之一。本研究以采自沙柳当年生健壮嫩枝茎段为研究对象，通过组织培养手段使其器官形成并分化，继而诱导继代形成再生植株，以探索其在不同组织培养条件下形成再生植株的关键技术。结果表明，沙柳在MS培养基上芽分化效果较好，适宜分化增殖培养基为MS + 0.5 mg/L 6-BA + 0.1 mg/L NAA；继代培养基为MS + 0.2 mg/L 6-BA + 0.2 mg/L KT + 0.1 mg/L NAA，并与激素稍高浓度的培养基MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA交替培养达到效果更佳；生根培养基为1/2MS + 0.3 mg/L IBA + 0.05 mg/L NAA，可获得到沙柳再生体系的建立，该方法不仅为沙柳稳定性遗传转化研究提供材料来源，也为今后实现沙柳工厂化育苗提供理论与技术依据。

Abstract: Salix psammophila is one of the important eco-economic plants in northern arid and desert region. In this experiment, on the basis of the strong tender stem segments, the key skills of how regene-rated plant forms in different Salix psammophila tissue culture conditions were explored, by means of tissue culture to get the organs formed and differentiated and to induce regeneration plants formed in succession. Research results show that Salix psammophila gets better bud differentiation effect in MS medium, the suitable differentiation and proliferation medium is MS + 0.5 mg/L 6-BA + 0.1 mg/L NAA; the subculture medium is MS + 0.2 mg/L 6-BA + 0.2mg/L KT + 0.1 mg/L NAA, with shifting the culture with the medium MS + 1.0 mg/L 6-BA + 0.1 mg/L NAA, a medium with little higher hormone, to achieve the best effect; the rooting medium is 1/2MS + 0.3 mg/L IBA + 0.05 mg/L NAA, all the results establish the regeneration system. This method not only provides the materials source for Salix psammophila stable genetic transformation studies, but also offers theoretical and technical support for Salix psammophila industrialized seeding production in the future.