山丹miR396a和miR396f表达载体构建及农杆菌的转化
Lilium pumilumu miR396a and miR396f Expression Vector Construction and the Transformation of Agrobacterium
DOI: 10.12677/BR.2017.66055, PDF, HTML, XML, 下载: 1,345  浏览: 5,041  科研立项经费支持
作者: 刘 红, 王文和, 张 克, 冷平生, 何祥凤：北京农学院，北京
关键词: 山丹；miR396；载体构建；农杆菌EHA105 ；Lilium pumilumu； miR396； Vector Construction； Agrobacterium EHA105
摘要: 为进一步探索miR396a和miR396f在百合中的基因功能和培育具有抗病性的百合，本试验首先从具有抗性的山丹中提取了它的的总DNA，利用PCR技术扩增到了山丹miR396a和miR396f前体片段，然后将获得的miR396a和miR396f前体片段通过BamHI酶和KpnI酶双酶切后,分别构建到含有Ubiquitin (Ubi)启动子的UBI-pCANBIA1301表达载体中，最后将构建好的载体转化到易侵染百合的农杆菌EHA105菌株中，以期为miR396基因家族在百合中的功能验证和抗病百合的培育奠定基础。
Abstract: In order to further explore the gene function of miR396a and miR396f in Lilium and to cultivate disease-resistant lily, we first extracted total DNA from Lilium pumilumu, then amplificated miR396a and miR396f precursor fragments using PCR technology. The miR396a and miR396f precursor fragments were digested with BamHI and KpnI, and then respectively constructed into UBI-pCANBIA1301 expression vector with Ubiquitin (Ubi) promoter. Finally, the constructed vector was transformed into Agrobacterium tumefaciens EHA105. This study will lay a foundation for the functional verification of miR396 family in lilium and the cultivation of disease-resistant lily.
文章引用：刘红, 王文和, 张克, 冷平生, 何祥凤. 山丹miR396a和miR396f表达载体构建及农杆菌的转化[J]. 植物学研究, 2017, 6(6): 422-428. https://doi.org/10.12677/BR.2017.66055

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