木犀草素对小球藻抑制作用的机理研究

Inhibitory Effect of Luteolin on Chlorella vulgaris

摘要: 为了探究木犀草素对小球藻抗氧化系统的影响和抑藻作用机理，进而考察不同浓度、不同作用时间下，木犀草素对小球藻细胞内丙二醛(MDA)含量，超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性的变化影响，通过扫描电镜观察藻细胞在木犀草素作用下的结构变化情况，结果表明，木犀草素随着浓度增加和时间的延长，其细胞内丙二醛(MDA)含量提高，超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性均降低。扫描电镜结果显示其首先破坏藻的细胞膜结构并最后导致其细胞破碎衰亡，其对小球藻的半抑制浓度IC50 = 10.68 mg/L (5 d, P < 0.05)。

Abstract: In this work, we explored the effect of luteolin on antioxidant system and the inhibition mechan-ism of Chlorella vulgaris. The effects of luteolin on the activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) and the content of malondialdehyde (MDA) in vivo were investigated under different concentration and different time. Scanning electron microscope was used to observe the structural changes of algal cells. The results showed that the content of malondialdehyde (MDA) in the cells increased with the increase of concentration and time, and the activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) decreased. The scanning electron microscopy (SEM) results showed that it first destroyed the cell membrane structure of the algae and finally resulted in the breakage and decay of its cells, and its semi inhibitory concentration of IC50 = 10.68 mg/L (5 d, P < 0.05).