摘要:
目的：研究甘草提取液和海尔福清千口服液(原海尔福口服液)对铅中毒小鼠的治疗效果。方法：提取、制备甘草提取液、海尔福清千口服液，造模前取血测定血红蛋白(Hb)；铅中毒模型的建立：除正常组外，其余各组用4 mg/ml乙酸铅溶液按14 mg/kg给小鼠腹腔注射，每日1次，直到实验结束；当造模2周后，治疗1组(甘草组)用甘草提取液0.2 ml/只，经口灌胃给小鼠灌服，每日一次至实验结束。治疗2组(清千组)用海尔福清千口服液0.2 ml/只，经口灌胃，给小鼠灌服，每天一次，连续二周。实验对照组(模型组)和正常对照组用相同量体积的纯净水灌胃；试验最后，抽取小鼠全血，静置，待血液凝固析出血清，离心，取血清测定GPT、GC、TC、尿素等；处死动物后取器、用甲醛浸泡后，制作切片，做镜下病理研究；取大脑，在冷浴下用0.9%氯化钠盐水制成10%脑匀浆，测定大脑各种指标，如 清除率。结果：正常组、模型组、甘草组、清千组测定结果依次排列；染毒前Hb分别为176.65 ± 13.32、181.73 ± 14.76、183.48 ± 19.30、196.04 ± 13.97 (g/L)，四组间比较分析，差异明显，有显著性；灌药前(造模后) Hb分别为175.88 ± 16.95、119.75 ± 16.99、123.23 ± 15.35、115.46 ± 24.14 (g/L)，染毒各组明显低于正常组，P < 0.01，染毒组明显下降；灌药后Hb分别为177.51 ± 10.30、121.12 ± 27.43、149.56 ± 9.75、152.04 ± 11.46 (g/L)，染毒各组仍低于正常组，P < 0.01，但灌药组已有明显上升，接近正常；TG分别为0.75 ± 0.15、1.22 ± 0.63、0.76 ± 0.20、0.56 ± 0.17 (mmol)，四组比较，差异明显，有统计学意义；TC分别为4.88 ± 0.31、6.10 ± 0.69、4.74 ± 1.03、4.17 ± 0.82 mmol四组比较，差异明显，有统计学意义；尿素氮分别为6.54 ± 0.91、8.09 ± 1.41、5.74 ± 1.21、6.02 ± 1.99 mmol，四组比较，差异明显，有统计学意义；血清GPT活力分别为8.00 ± 3.56、10.50 ± 5.30、8.00 ± 7.79、18.67 ± 7.24，GPT活力均在正常范围内，但清千组明显高于其他组；谷胱甘肽过氧化物酶(GSH-PX)活力分别为237.36 ± 56.21、321.04 ± 57.02、298.34 ± 104.76、288.30 ± 68.23，模型组活力较高；总蛋白(g/L)分别为46.63 ± 6.17、40.36 ± 5.94、43.94 ± 9.67、0.20 ± 7.47，总蛋白含量，清千组明显低于其他组。造模前、中、灌药后体重(g/只)统计结果，数据表明，染毒前，四组比较，差异不明显，无统计学意义。灌药前，四组比较，差异明显，有统计学意义，正常组体重明显增加，灌药组下降。灌药后，差异明显，有统计学意义，除清千组上升外，其他组均下降。结论：甘草提取液和海尔福清千口服液(原海尔福口服液)对铅染毒小鼠灌药治疗后，造血、肝、肾功能及脑抗氧化功能明显好转。
Abstract:
Objective: To study the therapeutic effect of licorice extract and Haerfuqing Thousand Oral liquid (original Haerfuqing oral liquid) on lead poisoning mice. Methods: Extract and prepare Glycyrrhiza uralensis extract and Hailfuqing Thousand Oral liquid, and take blood for hemoglobin (Hb) before modeling. Establishment of lead poisoning model: Except the normal group, the mice in other groups were intraperitoneally injected with 4 mg/ml lead acetate solution at 14 mg/kg, once a day until the end of the experiment. After 2 weeks of modeling, the treatment group 1 (the Glycyrrhiza uralensis group) was given 0.2 ml of Glycyrrhiza uralensis extract through oral gavage to mice, once a day until the end of the experiment. The treatment group 2 (the Qingqian group) was orally administrated with 0.2 ml of Hailfuqingqian oral solution to mice once a day until the end of the experiment. In the model group and the normal group, equal volume distilled water was used instead of irrigation, and the method was the same. At the end of the experiment, blood was collected from the eyeball of mice, serum was separated and ALT and urea were measured. After the animals were killed, the liver and kidney were taken and fixed with 10% formaldehyde, and the slices were made for pathological study under the microscope. The brain was taken and frozen to make 10% brain homogenate with normal saline to determine the free radical scavenging rate of brain. Results: The results of the normal group, the model group, the Licorice group and the Qingqian group were arranged in order. Before modeling, Hb was 176.65 ± 13.32, 181.73 ± 14.76, 183.48 ± 19.30 and 196.04 ± 13.97 (g/L), respectively. The difference of hemoglobin (Hb) between groups was statistically significant. After modeling (before administration), Hb was 175.88 ± 16.95, 119.75 ± 16.99, 123.23 ± 15.35, 115.46 ± 24.14 (g/L), which was significantly higher in the normal group than in other groups, P < 0.01, and significantly decreased in the modeling group. After administration, Hb was 177.51 ± 10.30, 121.12 ± 27.43, 149.56 ± 9.75, and 152.04 ± 11.46 (g/L), respectively. The normal group was significantly higher than other groups, P < 0.01, and the perfusion group significantly recovered. TG levels were 0.75 ± 0.15, 1.22 ± 0.63, 0.76 ± 0.20, and 0.56 ± 0.17 (mmol), respectively. TC was 4.88 ± 0.31, 6.10 ± 0.69, 4.74 ± 1.03, and 4.17 ± 0.82 mmol, respectively, with statistically significant differences. Urea nitrogen was 6.54 ± 0.91, 8.09 ± 1.41, 5.74 ± 1.21 and 6.02 ± 1.99 mmol, respectively. The differences were statistically significant. Serum GPT activity was 8.00 ± 3.56, 10.50 ± 5.30, 8.00 ± 7.79 and 18.67 ± 7.24, respectively. The activity of glutathione peroxidase (GSH-PX) was 237.36 ± 56.21, 321.04 ± 57.02, 298.34 ± 104.76, and 288.30 ± 68.23 in the model group. The total protein (G/L) was 46.63 ± 6.17, 40.36 ± 5.94, 43.94 ± 9.67 and 0.20 ± 7.47, respectively. The total protein content of the Qingqian group was significantly lower than that of other groups. Statistical results of body weight (g/only) before, during and after injection showed that there was no statistically significant difference between the groups before modeling. Before administration, the differences between groups were statistically significant, with significant increase in the normal group and decrease in other groups. After administration, the difference was statistically significant, with an increase in the Qingqian group and a decrease in other groups. Conclusion: Hematopoietic, liver, kidney, function and brain antioxidant function of lead-infected mice were significantly impaired, and were significantly improved after administration.