不同品种桃果实中内切多聚半乳糖醛酸酶EndoPG基因的表达分析
Expression Analysis of EndoPG Genes in Peach Fruits of Different Cultivars
DOI: 10.12677/BR.2018.74056, PDF,   
作者: 刘佩卓, 谷超：南京农业大学园艺学院，江苏 南京
关键词: 内切多聚半乳糖醛酸酶；桃；果实软化；基因表达；Endopolygalacturonase； Peach； Fruit Softening； Gene Expression
摘要: 内切多聚半乳糖醛酸酶(endopolygalacturonase, EndoPG)是一种重要的细胞壁水解酶，在果实的成熟软化过程中具有重要作用。为探究桃果实软化的分子机制，本研究从桃全基因组中分离出6个EndoPG基因EndoPG1、EndoPG2、EndoPG3、EndoPG4、EndoPG5和EndoPG6，以不同桃品种“南山甜桃”、“朝晖”和“明星”果实为研究材料，通过实时荧光定量分析了这6个EndoPG基因在果实发育过程中的表达情况。研究结果显示：6个EndoPG基因在“南山甜桃”和“朝晖”的果实发育过程中均有表达，其中EndoPG3/4基因表达水平显著；在“明星”中，仅检测到4个EndoPG基因EndoPG1、EndoPG2、EndoPG5和EndoPG6的表达。结果表明EndoPG基因在桃果实成熟过程中发挥了重要作用。本研究为进一步探究内切多聚半乳糖醛酸酶调控桃果实成熟软化的分子机制奠定了基础。
Abstract: Endopolygalacturonase is an important cell wall hydrolase, which plays an important role in the ripening and softening of fruits. In order to explore the molecular mechanism of peach fruit sof-tening, six EndoPG genes, EndoPG1, EndoPG2, EndoPG3, EndoPG4, EndoPG5 and EndoPG6, were iso-lated from the whole genome of peach. Different peach cultivars “Nanshantiantao”, “Zhaohui” and “Myojo” were chosen as experiment materials to compare the expression level of six EndoPG genes during fruit development by real-time fluorescence quantitative analysis. The results showed that six EndoPG genes expressed in “Nanshantiantao” and “Zhaohui” during fruit development. Specially, EndoPG3/4 gene showed a significant level. In the fruit of “Myojo”, only four EndoPG genes, EndoPG1, EndoPG2, EndoPG5 and EndoPG6 were detected. The results show that the EndoPG gene plays an important role in the fruit ripening of peach. This study laid the foundation for further exploring the molecular mechanism of peach fruit ripening and softening regulation of endopolygalacturonase.
文章引用：刘佩卓, 谷超. 不同品种桃果实中内切多聚半乳糖醛酸酶EndoPG基因的表达分析[J]. 植物学研究, 2018, 7(4): 463-470. https://doi.org/10.12677/BR.2018.74056

参考文献

[1] 索标. 桃果实软化过程中细胞壁多糖降解特性的研究[D]: [硕士学位论文]. 扬州: 扬州大学, 2006.
[2] 麻国升. 桃果实肉质、粘离核及硬度的关联分析[D]: [硕士学位论文]. 北京: 中国科学院, 2012.
[3] 孙升. 杏, 李资源粘离核分级标准的探讨[C]//全国李杏资源研究与利用学术交流会. 2000.
[4] 陈昆松, 张上隆, 吕均良, 沈淦卿, 林意珠. “玉露”桃不同采收成熟度的贮运性及其生理基础研究[J]. 浙江农业学报, 1993, 5(4): 237-239.
[5] John, M.A. and Dey, P.M. (1986) Postharvest Changes in Fruit Cell Wall. Advances in Food Research, 30, 139-193. [Google Scholar] [CrossRef] 
[6] 薛炳烨, 束怀瑞. 多聚半乳糖醛酸酶(PG)与果实成熟软化研究进展[J]. 山东农业大学学报, 2002, 33(2): 252-256.
[7] PhaffH, J. and Demain, A.L. (1956) The Unienzymatic Nature of Yeast Polygalac-turonase. Journal of Biological Chemistry, 218, 875.
[8] Crooks, P.R. and Grierson, D. (1983) Ultrastructure of Tomato Fruit Ri-pening and the Role of Polygalacturonase Isoenzymes in Cellwall Degradation. Plant Physiology, 72, 248-254.
[9] Brummell, D.A. and Harpster, M.H. (2001) Cell Wall Metabolism in Fruit Softening and Quality and Its Manipulation in Transgenic Plants. Plant Molecular Biology, 47, 311-340. [Google Scholar] [CrossRef] 
[10] Quesada, M.A., Blanco-Portales, R., Posé, S., García-Gago, J.A., Jiménez-Bermúdez, S., Muñoz-Serrano, A., Caballero, J.L., Pliego Alfaro, F., Mercado, J.A. and Muñoz-Blanco, J. (2009) Antisense Downregulation of the FaPG1 Gene Reveals an Unexpected Central Role for Polygalacturonase in Strawberry Fruit Softening. Plant Physiology, 150, 1022-1032. [Google Scholar] [CrossRef] [PubMed]
[11] Tacken, E., Ireland, H., Gunaseelan, K., Karunairetnam, S., Daisy, Z.W., Keith, S., Bowen, J., Atkinson, G.R., Johnston, J., Putterill, J., Hellens, R. and Schaffer, J.R. (2010) The Role of Ethylene and Cold Temperature in the Regulation of the Apple Polygalacturonase1 Gene and Fruit Softening. Plant Physiology, 153, 294-305. [Google Scholar] [CrossRef] [PubMed]
[12] Leida, C., Ríos, G., Soriano, J.M., Pérez, B., Llácer, G., Crisosto, C.H. and Badenes, M.L. (2011) Identification and Genetic Characterization of an Ethylene-Dependent Polygalacturonase from Apricot Fruit. Postharvest Biology & Technology, 62, 26-34. [Google Scholar] [CrossRef] 
[13] Prasanna, V. and Prabha, T.N. (2007) Tharanathan, R.N. Fruit Ripening Phenomena—An Overview. Critical Reviews in Food Science & Nutrition, 47, 1-19. [Google Scholar] [CrossRef] [PubMed]
[14] Tomassen, M.M., Barrett, D.M., van der Valk, H.C. and Woltering, E.J. (2007) Isolation and Characterization of a Tomato Non-Specific Lipid Transfer Protein Involved in Polygalacturonase-Mediated Pectin De-gradation. Journal of Experimental Botany, 58, 1151-1160. [Google Scholar] [CrossRef] [PubMed]
[15] Livak, K.J. and Schmittgen, T.D. (2001) Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-ΔΔCtMethod. Methods, 25, 402-408. [Google Scholar] [CrossRef] [PubMed]
[16] Chao, G., Lu, W., Wei, W., Hui, Z., Baiquan, M., Hongyu, Z., Ting, F., Collins, O., Sornkanok, V. and Yuepeng, H. (2016) Copy Number Variation of a Gene Cluster Encoding Endopolygalacturonase Mediates Flesh Texture and Stone Adhesion in Peach. Journal of Experimental Botany, 67, 1993-2005. [Google Scholar] [CrossRef] [PubMed]
[17] 彭丽桃, 饶景萍, 杨书珍, 任小林, 王俊宁. 果实软化的胞壁物质和水解酶变化[J]. 热带亚热带植物学报, 2002, 10(3): 271-280.
[18] 赵云峰, 林河通, 林娇芬, 陈绍君. 果实软化的细胞壁降解酶及其调控研究进展[J]. 仲恺农业技术学院学报, 2006, 19(1): 65-70.