不同提取方法对山药多糖提取率及抗氧化活性的影响

Effects of Different Extraction Methods on the Yield and Antioxidant Activities of Rhizoma dioscoreae Polysaccharides

摘要: 本文研究不同提取方法对山药多糖提取率及抗氧化活性的影响。采用热水回流、超声波辅助、热水–酶联合以及超声波–酶联合四种方法提取山药多糖。通过提取率测定、电镜扫描、抗氧化测定对四种提取方法的提取效果和样品活性进行评价。上述四种提取方法的提取率分别为0.465%、0.851%、0.443%、1.929%。超声波–酶联合方法提取获得多糖抗氧化活性强于其他三种方法。结果表明，超声波–酶联合法为最佳提取方法，同时山药多糖表现出较强的抗氧化能力，这些都为后续相关药物或功能性食品的开发奠定了重要基础。

Abstract: In this paper, the effects of different extraction methods on yield and antioxidant activity of polysaccharides from Rhizoma dioscoreae were investigated. Four different extraction methods, including hot water reflux, ultrasonic assisted, hot water enzyme combination and ultrasonic en-zyme combination, were used to extract Rhizoma dioscoreae polysaccharides. The extraction efficiency and antioxidant activity of the four extraction methods were evaluated by extraction rate, electron microscopy and antioxidant. The yields of the above four extraction methods are 0.465%, 0.851%, 0.443%, 1.929% respectively. The antioxidant activity of polysaccharide extracted by ultrasonic enzyme combination method was better than that of the other three methods. The results showed that ultrasonic enzyme combination is the best extraction method, and the Rhizoma dioscoreae polysaccharides have strong antioxidant activity, which lay an important foundation for the development of the follow-up related drugs or functional foods.