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徐大文, 马海伦, 任中原, 等. 苦味叶下珠抗乙型肝炎病毒的研究[J]. 天津医药, 1994, (12): 717-721.

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  • 标题: HPLC测定扶正柔肝颗粒柯里拉京和丹酚酸B的含量Determination of Corilagin and Salvianolic Acid B in Fu Zheng Rou Gan by HPLC

    作者: 宋力飞, 刘常青, 李曼莎, 阳建军, 李常青

    关键字: 扶正柔肝颗粒, 含量测定, 柯里拉京, 丹酚酸B, HPLCFu Zheng Rou Gan, Content Determination, Corilagin, Salvianolic Acid B, HPLC

    期刊名称: 《Pharmacy Information》, Vol.4 No.4, 2015-11-27

    摘要: 目的:建立高效液相色谱法(HPLC)测定复方扶正柔肝颗粒中柯里拉京和丹酚酸B含量的方法。方法:色谱柱:InertsiL® ODS-SP (5 μm, 4.6 × 250 mm);流动相:乙腈-0.2%磷酸(梯度洗脱);检测波长为210 nm;流速为1.0 mL/min;柱温35℃;进样量5 μL。结果:柯里拉京在0.061~0.510 μg的范围内线性关系良好(r = 0.9997),平均加样回收率为101.41% (RSD = 1.68%, n = 6);丹酚酸B在0.504~4.203 μg的范围内(r = 0.9999)线性关系良好,平均加样回收率为103.30% (RSD = 1.11%, n = 6)。结论:本法灵敏、快速、准确,可用于复方扶正柔肝颗粒的质量控制。Objective: To establish a high liquid chromatography method of determinating Corilagin and Sal-vianolic acid B in Fu Zheng Rou Gan. Method: Chromatographic column: InertsiL® ODS-SP (5 microns, 4.6 × 250 mm). The mobile phaseis is acetonitrile-0.2% phosphoric acid (gradient elution) with detection wavelength 210 nm; the flow rate is 1.0 mL/min; the column temperature is 35˚C and the inject volume is 5 μL. Result: In the range of 0.061 - 0.510 μg, the linear relationship of Corilagin is good (r = 0.9997), and the average sample recovery rate is 101.41% (RSD = 1.68%, n = 6). In the range of 0.504 - 4.203μg, the linear relationship of Salvianolic acid B is also good (r = 0.9999), and the average sample recovery rate is 103.30% (RSD = 1.11%, n = 6). Conclusion: The method is sensitive, rapid and accurate and can be used for quality control of this granule.

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