标题:
2个芒果品种果实对炭疽病的抗性差异及其相关生理基础The Difference in Resistance of Two Mango Cultivars to Anthracnose Disease and Its Physiology Mechanism Involved
作者:
弓德强, 黄光平, 邓立宝, 李雄辉, 王晓, 梁清志, 朱世江
关键字:
芒果, 抗病性, 炭疽病, 防御酶, 抗性物质Mango, Disease Resistance, Anthracnose, Defense Enzymes, Antifungal Substance
期刊名称:
《Hans Journal of Agricultural Sciences》, Vol.5 No.6, 2015-12-29
摘要:
2个芒果品种果实对炭疽病的抗性差异及其相关生理基础，为利用抗病性诱导技术防治芒果采后病害提供理论依据。方法：以两个芒果品种“吉禄(Zill)”和“凯特(Keitt)”为试材，通过人工接种和未接种芒果炭疽菌的方式，比较2个品种芒果采后抗病性的差异，并测定了两个芒果品种在果实发育和贮藏过程中苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和多酚氧化酶(PPO)等防御酶的活性以及过氧化氢(H2O2)、总酚和木质素等次生抗菌物质含量的变化。结果：对接种炭疽菌的芒果果实，“凯特”芒果的病斑直径显著小于“吉禄”芒果，对未接种炭疽菌的芒果果实，“凯特”芒果的病情指数显著小于“吉禄”芒果，表明“凯特”芒果果实对炭疽病的抗性强于“吉禄”芒果；与“吉禄”芒果相比，在果实发育和贮藏过程中“凯特”芒果果实有较高的苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和多酚氧化酶(PPO)等防御酶的活性以及过氧化氢(H2O2)、总酚和木质素等次生抗菌物质的含量。结论：“凯特”芒果对炭疽病的抗性强于“吉禄”芒果，并且这些防御酶和抗性物质可能在芒果果实对 炭疽菌侵染的抗性反应中起着重要的作用。
Objectives: The difference in resistance of two
mango cultivars to anthracnose and the possible physiology
mechanism involved were studied to provide theoretical references for
controlling postharvest diseases of mango fruit by using the technology of
induced disease resistance. Methods: The difference in the resistance between
two mango cultivars (“Zill” and “Keitt”) to postharvest disease was examined by
inoculation or non-inoculation with pathogen Colletotrichum gloeosporioides, and the defense enzyme activities of phenylalanine ammonia lyase (PAL),
peroxidase (POD), and polyphenol oxidase (PPO) and the contents of hydrogen
peroxide (H2O2), total phenolic compounds and lignin during
fruit development and storage were determined. Results: The results showed that
in fruits inoculated with C. gloeosporioides, the lesion size on “Keitt”
fruit was smaller than that on “Zill”; in non-inoculated fruits, the disease
index of “Keitt” was lower than that of “Zill”. This indicated “Keitt” was more
disease resistant than “Zill”. Compared with “Zill”, “Keitt” showed higher enzymatic
activities of PAL, POD, and PPO and the accumulation of more H2O2,
total phenolic compounds and lignin content in fruits during fruit development
and storage. Conclusions: These results suggest that “Keitt” is more disease
resistant than “Zill” to anthracnose and the defense enzymes and antifungal
substance probably play an important role in the resistance of mango fruits to anthracnose
infection.