标题:
高效液相色谱法测定海藻油中的DHA甲酯Determination of DHA-ME in Seaweed Oil by HPLC
作者:
蔡凤珊, 张琳, 吴世凯
关键字:
DHA甲酯, 海藻油, 高效液相色谱DHA-ME, Seaweed Oil, HPLC
期刊名称:
《Bioprocess》, Vol.6 No.1, 2016-03-11
摘要:
目的：建立高效液相色谱法分离测定海藻油样品中的二十二碳六烯酸甲酯(DHA甲酯)含量的方法。方法：采用Agilent Eclipse XDB-C18色谱柱(150 mm × 4.6 mm, 5 μm)，以乙腈-水(92:8)溶液为流动相，流速为1.0 mL/min，检测波长205 nm。结果：DHA甲酯在10~500 μg/mL的范围内呈良好线性，线性回归方程为y = 2.586 × 103x + 5.927 × 103 (r2 = 1)，平均回收率为94.23%，相对标准偏差为7.01%，海藻油中DHA-甲酯的平均含量为7.3876 mg/g。结论：本检测方法简便、快速、重现性好，可用于海藻油样品中DHA甲酯含量的测定。
A method was developed for the determination of methyl-DHA in seaweed oil by high performance liquid chromatography (HPLC). The HPLC separation conditions were as follows: an Agilent Eclipse XDB-C18 column (150 mm × 4.6 mm, 5 μm) and elution with acetonitrile-water (v:v = 92:8), UV dection wavelength at 205 nm, 1.0 mL/min of flow rate for mobile phases. On the above conditions, good linearity was found over a concentration range of 10 - 500 μg/mL for methyl-DHA. Linear regression equation was y = 2.586 × 103x + 5.927 × 103 (r2 = 1). The average recovery rate was 94.23% (RSD = 7.01%). The average detection of methyl-DHA in seaweed oil was 7.3876 mg/g. This method was simple, rapid and high reproducibility; it can be used for the detection of methyl-DHA in seaweed oil.