不同仪器测定化妆品中氯霉素的含量Different Instrument for Determining the Content of Chloramphenicol in Cosmetics
陈科, 刘翠红, 李练培
超高效液相色谱仪, 二极管阵列检测器, 祛痘除螨类化妆品, 氯霉素Ultra High Performance Liquid Chromatograph, Diode Array Detector, Cosmetics for Removing Acne and Acarus, Chloramphenicol
《Advances in Analytical Chemistry》, Vol.6 No.3, 2016-08-25
目的：采用三种不同液相色谱仪，对祛痘除螨类化妆品中氯霉素含量进行测定。方法：在相同的实验条件下，以氯霉素为标准物质，C18柱(250 mm × 4.6 mm, 5 µm)为分离柱，(甲醇 + 乙腈 = 1:2):0.01 mol/L草酸(pH = 2.0) = 40：60为流动相洗脱，二极管阵列检测器，检测波长268 nm，流速：0.8 mL/min。结果：L-3000、LC-20AT、L-6000测定的线性相关系数r分别为0.9976、0.9994、0.9998，精密度的保留时间与峰面积RSD%依次分别为1.8%和2.1%、0.85%和0.91%、0.50%和0.72%，稳定性RSD%分别为2.3%、0.37%、0.16%，重复性RSD%分别为2.7%、0.86%、0.57%，回收率和RSD%依次分别为96.2%、2.2%；97.4%、1.3%；99.3%、0.7%。结论：经比较，使用超高效液相色谱仪测定氯霉素灵敏度低、精密度高、重复性好。
Objective: Using three different liquid chromatographs, to determine the chloramphenicol content in cosmetics for removing acne and acarus. Methods: Under the same experimental conditions, on the basis of chloramphenicol material, C18 column (250 mm × 4.6 mm, 5 µm) was used as the sep-aration column, (methanol + acetonitrile = 1:2):0.01 mol/L oxalic acid (pH = 2.0) = 40:60 as mobile phase elution, and diode array detector was also used with detection wavelength of 268 nm, velocity of 0.8 mL/min. Results: The linear correlation coefficient r of L-3000, LC-20AT, L-6000 was 0.9976, 0.9994, 0.9998 respectively; RSD% of precision retention time and peak area were 1.8% and 2.1%, 1.8% and 0.91%, 0.50% and 0.72%, respectively; RSD% of stability was 2.3%, 0.37% and 2.3% respectively; RSD% of repetitive peak area was 2.7%, 0.86% and 2.7% respectively; the recovery rate and RSD% were 96.2% and 96.2%, 97.4% and 1.3%, 99.3% and 0.7% respectively. Conclusion: After comparing, using ultra performance liquid chromatography to determine chloramphenicol has advantages of low sensitivity, high precision and good repeatability.