标题:
佛山白秃马勃化学成分预分析与抗氧化活性Chemical Constituents Pre-Analysis and Antioxidant Activity of the Puffball Calvatia candida from Foshan
作者:
吴静姝, 朱峰
关键字:
白秃马勃, 高等真菌, 化学成分, 试管预试法, 抗氧化活性Calvatia candida, Higher Fungus, Chemical Constituent, Pre-Test Tube Method, Antioxidant Activity
期刊名称:
《Journal of Organic Chemistry Research》, Vol.4 No.3, 2016-09-22
摘要:
在综述秃马勃属真菌化学成分研究的基础上，通过多种指示剂和显色剂的显色反应或沉淀反应对佛山白秃马勃化学成分进行了预分析，并通过羟自由基(•OH)和1,1-二苯基-2-苦肼基(DPPH)清除试验评价了其甲醇提取物的体外抗氧化活性。结果表明，佛山白秃马勃含有生物碱、甾体和萜类化合物，具有有效的抗氧化活性，对•OH和DPPH自由基的半数清除浓度(IC50)分别为241.55和418.63 μg/mL。
Based on review of chemical composition of the genus of Calvatia sp., the chemical constituents of the puffball Calvatia candida from Foshan were analyzed by pre-test tube method, and its antioxi-dant activities were evaluated by the biochemical assay of hydroxyl and DPPH radicals scavenging activity in vitro. The results show that, the puffball Calvatia candida from Foshan contain alkaloids, steroids and terpenoids, and have potent antioxidant activities with IC50 values of 241.55 (for •OH) and 418.63 (for DPPH) μg/mL, respectively.