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粒毛盘菌多酚理化特征及其生物活性研究Physicochemical Characteristics and Biological Activities of Polyphenols from Lachnum
钱梅双, 纪静, 陈天乐, 杨柳, 叶明
粒毛盘菌, 胞外多酚, 理化特征, 抗氧化活性, 抑菌活性Lachnum; Extracellular Polyphenols; Physicochemical Characteristics; Antioxidant Activity; Antibacterial Activity
《Advances in Microbiology》, Vol.2 No.4, 2013-12-24
本文作者提取并纯化了粒毛盘菌YMU50胞外多酚(LTEP），研究了其理化特征并测定其抗氧化活性及抑菌活性。紫外光谱显示其在270 nm和340 nm处有最大吸收峰，表现出典型的酚类特征；红外光谱显示其在3354.360 cm-1和1048.762 cm-1处含有酚类的特征吸收峰。抗氧化试验表明，当LTEP浓度为2 mg/mL时，对、DPPH、·OH和的清除率分别为76.43%、72.7%、55.56%、59.4%；抑菌试验显示，LTEP对金黄色葡萄球菌、酿酒酵母、大肠杆菌、伤寒沙门氏菌均具有明显抑制作用，四种菌的最低抑菌浓度(MIC)分别为0.05、0.1、0.1、0.2 mg/mL，结果表明LTEP具有较强的抗氧化活性和抑菌活性。
Lachnum YMU50 total extracellular polyphenols (LTEP) were extracted and purified. The physicochemical properties, antioxidant and antibacterial activities of LTEP were investigated. UV spectroscopy analysis indicated that LTEP had typical characteristics absorption peaks of phenolic at 270 nm and 340 nm. IR spectroscopy displayed that LTEP had characteristics absorption peaks of phenolic at 3354.360 cm-1and 1048.762 cm-1. Antioxidant trials showed that the scavenging effect of LTEP on , DPPH, ·OH and were 76.43%, 72.70%, 55.56% and 59.40% respectively, at the 2 mg/mL concentration of LTEP. LTEP also showed a significantly antibacterial activity against Staphylococcus aureus, S. cerevisiae, E. coli, and Salmonella typhi. The MIC (minimum inhibitory concentration) of these four strains was 0.05, 0.1, 0.1 and 0.2 mg/mL, respectively. The results revealed that LTEP had strong antioxidant and antibacterial activities.